面积校正归―法定量分析α蒎烯、β蒎烯、对伞花烃和长叶烯.pdfVIP

面积校正归一法定量分析α-蒎烯、β-蒎烯、 对伞花烃和长叶烯 莫伟华，陈小鹏，王琳琳，祝远姣，张志丽，陆梦婕 (广西大学化学化工学院，广西 南宁 530004) 摘要：使用GC-1102 型气相色谱仪，FID 检测器，SIMPLCITY-5 石英玻璃毛细管柱（30 m ×0.32 mm ×0.25 μm ），采用 面积校正归一法对样品中的α-蒎烯、β-蒎烯、对伞花烃和长叶烯进行了定量分析。结果表明，α-蒎烯、β-蒎烯和长叶烯相 对于对伞花烃的相对质量校正因子分别为：1.0891，1.0879，1.0700。考察了方法的精密度和回收率，4 种组分的相对标 准偏差（RSD ）分别为：0.78% ，0.59% ，1.04%，2.62% 。加标回收率分别为：99.52%，101.91%，100.97%，100.70%。 比较了面积校正归一法和面积归一法分析结果与实际值的相对偏差，两者的分析结果与实际值的最大相对偏差分别为 1.59%和4.43% 。 关键词：蒎烯；对伞花烃；长叶烯；定量分析 Quantitative analysis of α-Pinene , β-Pinene , p- Cymene and longifolene by area correction normalization method MO Weihua ，CHEN Xiaopeng ，WANG Linlin ，ZHU Yuanjiao ，ZHANG Zhili ，LU Mengjie (College of Chemistry and Chemical Engineering, Guangxi University , Nanning 530004 , Guangxi, China) Abstract ：α-Pinene , β-Pinene , p- Cymene and longifolene were determined using area correction normalization method by GC-1102 gas chromatography with FID detector and SIMPLCITY-5 capillary column(30 m ×0.32 mm ×0.25 μm). The results showed that the relative mass correction factors compared with p- Cymene were: 1.0891 ，1.0879，1.0700, respectively. The accuracy and recovery were tested, and the relative standard deviation (RSD) of the four components were 0.78% ，0.59% ，1.04%，2.62% , the recovery of standard addition were: 99.52%，101.91%，100.97%，100.70%, separately. The relative deviation of two methods was compared, the maximum relative error of two methods analysis value comparing actual were 1.59% and 4.43%. Key words : pinene ; p- Cymene ; longifolene ; quantitative analysis 引 言 组成为α-蒎烯、β-蒎烯、对伞花烃等单萜烯以 及长叶烯等倍半萜，且组成随着树种、气候条件