天麻中天麻素RP-HPLC测定及提取工艺研究 Determination of Gastrodin in Gastrodia elata B1. by Reverse Phase (RP)-HPLC and Study on Its Extraction Technology.pdfVIP

※工艺技术 食品科学 77 田洋1，肖蓉：一，徐昆龙2，史崇颖1，代神2 (1．云南农业大学食品科学技术学院，云南昆明 650201；2．云南农业大学动物科学技术学院，云南昆明650201) 摘 要：通过考察不同检测波长、流动相和溶剂对天麻素出峰效果的影响，改进了2005年版《中国药典》收载 的天麻药材中天麻素含量测定的RP．HPLC方法，并探讨了提取溶剂、提取温度、料液比、提取时间对天麻素提 取工艺的影响。结果表明：采用Diamonsil X Cls柱(150mm 关键词：天麻；天麻素；RH—HPLC；提取工艺 DeterminationofGastrodininGastrodiaelata Reverse and on B1．by Phase(RP)一HPLCStudy ItsExtraction Technology TIAN Yi2 Yan91，XIAORon92t+，XUKun—lon92，SHIChong—yin91，DAI ofFoodScienceand 650201，China； (1．College Technology，YunnanAgriculturalUniversity，Kunming ofAnimalScienceand 2．CoHege Technology，YunnanAgriculturalUuiversity，Kunming650201，China) theeffectsof differentdetection andsolventonthe of Abstract：Byinvestigating wavelength，mobilephase peakprofile thetraditionalRP—HPLCmethodfor in elata Gast^odiaB1．．Also， gastrodin，thepresentstudyimproved determininggastrodin theeffectsofextraction ratioandextractiontimeonthe rateof solvent，extraction temperature，solid-liquid gastrodin expaction werediscussed．Themsulmshowedthatthe conditionsofRP-HPLCwereas DiamonsilClscolumn optimalworking follows：using X rain4．6 ambient