【生物课件】An Algorithmic Approach to Peptide Sequencing via Tandem Mass .pptVIP

下载本文档

1
0
约3.47万字
约 75页
2017-12-12 发布于贵州
举报
版权申诉

【生物课件】An Algorithmic Approach to Peptide Sequencing via Tandem Mass .ppt

1、本文档共75页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

【生物课件】An Algorithmic Approach to Peptide Sequencing via Tandem Mass

An Algorithmic Approach to Peptide Sequencing via Tandem Mass Spectrometry Ming-Yang Kao Department of Computer Science Northwestern University Evanston, Illinois U. S. A. Collaborators of This Project Perspectives A key goal of bioinformatics: To study biological systems based on global knowledge of genomes, transcriptomes, and proteomes. Genome: entire sets of materials in the chromosomes. Transcriptome: entire sets of gene transcripts. Proteome: entire sets of proteins. Perspectives A key goal of bioinformatics: To study biological systems based on global knowledge of genomes, transcriptomes, and proteomes. Genome: entire sets of materials in the chromosomes. Transcriptome: entire sets of gene transcripts. Proteome: entire sets of proteins. Proteomics Proteome: all proteins encoded within a genome half millions distinct proteins (temporal, spatial, modifications) ~30,000 human genes mRNA and protein expressions may not correlate Proteomics: study of protein expression by biological systems relative abundance and stability; post-translational modifications fluctuations as a response to environment and altered cellular needs correlations between protein expression and disease state protein-protein interactions, protein complexes Technologies: 2D gel electrophoresis mass spectrometry yeast two-hybrid system protein chips A Key Step of Proteomics How to sequence proteins? How to sequence protein peptides? (this talk’s focus) Outline of This Talk Outline of This Talk (1) Protein Identification: HPLC-MS-MS Protein Identification: HPLC-MS-MS Peptide Fragmentation and Ionization B-ions and Y-ions Tandem Mass Spectrum Raw Tandem Mass Spectrum Prediction from Raw Tandem Mass Spectrum Protein Database Search Find the peptide sequences in a protein database that optimally fit the spectrum. It does not work if the target peptide sequence is not in the database. It does not work if there is an unknown modification at some amino acid. It is very slow because it must sea