愈创木酚生产菌.docx

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愈创木酚生产菌

That is, the present invention provides the following detection methods (1) to (6), detection kits (7) to (13), and methods for rapid determination of proliferation properties (14) to (17). (1) A method for detecting bacteria capable ofproducingguaiacol, which comprises culturing or incubating a culture solution containing a specimen for a predetermined time in the presence of vanillic acid, and measuring the produced guaiacol by qualitative or quantitative analysis. (2) The methoddescribed in (1), wherein said produced guaiacol is measured by GC-MS analysis or color reaction. (3) The method described in (1) or (2), wherein the color reaction for guaiacol employs an oxidation reaction using peroxidase as a catalyst and isdetected by spectrophotometry or visual observation. (4) The method described in any one of (1) to (3), wherein said bacteria capable of producing guaiacol to be detected are thermotolerant acidophilic bacteria and are cultured or incubated under anacid condition. (5) The method described in any one of (1) to (3), wherein said bacteria capable of producing guaiacol to be detected are bacteria belonging to the genus Bacillus and are cultured or incubated under a neutral condition. (6) The methoddescribed in (4), wherein said thermotolerant acidophilic bacteria are of A. acidoterrestris. (7) A kit for detection of bacteria capable of producing guaiacol, comprising a container, and an acid or neutral culture medium containing a vanillicacidsubstrate for production of guaiacol, which is contained in the container and also sterilized and sealed. (8) A kit for detection of bacteria capable of producing guaiacol, comprising a combination of the kit described in (7) and a means for detectionofguaiacol in combination. (9) The kit described in (7) or (8), wherein said means for detection of guaiacol is based on color reaction and includes a combination of at least hydrogen peroxide water, a peroxidase enzyme and a buffer for enzymaticreaction. (10) The kit fo

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