缺血后处理对大鼠脑缺血再灌注损伤的影响-中华医学会麻醉学分会.docVIP

缺血后处理对大鼠脑缺血再灌注损伤的影响 郝宇华 郭永清 吕国义 高春霖 【摘要】 目的 探讨缺血后处理对大鼠脑缺血再灌注损伤的影响。方法 成年雄性SD大鼠40只，体重250-300g，随机分为5组（n=8），①假手术组（sham组）：只进行麻醉和分离解剖两侧颈总动脉，②缺血再灌注1组（I/R1组）：给予脑缺血30min，再灌注24h，③缺血再灌注2组（I/R2组）：给予脑缺血30min，再灌注48h，④缺血后处理1组（Post1组）：给予脑缺血30min，缺血后处理后再灌注24h，⑤缺血后处理2组（Post2组）：给予脑缺血30min，缺血后处理后再灌注48h。使用无创动脉夹夹闭双侧颈总动脉实现脑缺血，撤去动脉夹实现再灌注。在再灌注即刻给予大鼠双侧颈总动脉松夹15s /夹闭15s，如此三次，实现缺血后处理。实验结束处死大鼠，开颅取左侧半球脑组织顶叶皮质制作脑组织匀浆，检测MDA含量和SOD活性。取右侧半球脑组织并作石蜡切片，采用TUNEL法观察大脑顶叶皮质细胞凋亡的情况。结果 与sham组相比，I/R1组、I/R2组、Post1组、Post2组MDA含量增高，SOD活性降低（P0.05）0.05）0.05）0.05）0.05）0.05）The effects of postconditioning on cerebral ischemic/reperfusion injury in rat cerebrum HAO Yu-hua*, LV Guo-yi, GAO Chun-lin, et al. Department of anesthesia, Shanxi People′s Hospital, Shanxi 030001, China 【Abstract】Objective To investigate the effects of postconditioning on brain apoptosis of the SD rat induced by brain ischemic/reperfusion and its mechanism. Brain tissue homogenate were collected to measure the content of MDA and the activity of SOD. Discussing the brain-protective mechanism of postconditiong and the potential clinical application. Methods Forty male SD rats weighting 250-300g were anesthetized by 10% chloral hydrate peritoneal injection. Rats were randomly divided into five groups：(1)The sham group; (2)ischemic/reperfusion 1 group：brains were subjected to 30mins of ischemia followed by 24 hours of reperfusion(I/R1 group);(3)ischemic/reperfusion 2 group： brains were subjected to 30mins of ischemia followed by 48 hours of reperfusion(I/R2 group);(4) postconditioning 1 group：brains were subjected to 30mins of ischemia followed by three circles of 15s/15s’postconditioning and 24 hours of reperfusion(Post1 group);(5) postconditioning 2 group: brains were subjected to 30 mins of ischemia followed by three circles of 15s/15s’postconditioning and 24 hours of reperfusion(Post2 group). All of the groups were assigned 8 rats. Bilateral common carotid artery were dissociation and occlusion for 30min followed by reperfusion or reperfusion p