SD大鼠骨髓间充质干细胞体外原代培养及鉴定.pdfVIP

421 现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.3 FEB.2011 · · SD 大鼠骨髓间充质干细胞体外原代培养及鉴定 * 何凤璞 汪黎明 陈 鑫△ （南京医科大学附属南京第一医院 南京市心血管病医院 江苏 南京 210006） 摘要 目的：探讨骨髓间充质干细胞(BMSCs)体外分离培养以及扩增 的方法并鉴定 。方法：取 100 g 左右雄性 SD 大鼠后肢股骨、胫 cd34 cd90 cd105 骨骨髓，原代全骨髓培养法 ，多次传代纯化 ，体外扩增后 ，观察细胞形态，并免疫组化及流式细胞仪检测 、 、 细胞因 BMSCs CD34 CD90 CD105 子，鉴定是否为 。结果：所获取的细胞呈长梭形 ，呈现特征性的漩涡状生长 ， 阴性 ， 、 阳性 。结论：利用全 10 骨髓培养法成功分离骨髓间充质干细胞， 代以内 的细胞纯度高 ，活性好 。全骨髓培养较为简便 、易行 。 关键词 ：骨髓 ；干细胞 ；细胞培养 Q95-3 R654.3 A 1673-6273 2011 03-421-03 中图分类号： ， 文献标识码： 文章编号： （ ） Study on Isolation, Culture and Identification of Bone Mesenchymal Stem Cells of SD Rats in Vitro* HE Feng -pu, WANG Li-ming, CHEN Xin△ (Dep artment of Thoracic and Cardiovascular Surgery, Nanj ing First Hosp ital Affiliated to Nanj ing Medical University, Nanj ing 2 10006, China) ABSTRACT Obj ective: To investigate the method for isolation and amplification of the bone mesenchymal stem cells (BMSCs) in vitro and to identified it. Methods: Male SD rats around 100g, the bone marrow cells were obtained from hind femur and tibia, whole bone marrow primary culture, purified by repeated passage in vitro amplification. Cell morphology was observed, and expressions of CD34, CD90, CD105 cell factors were examined by flow cytometry, to identify whether its the BMSCs. Results: Cells appeared spin- dle-shaped and showed characteristic swirling growth. The