解毒化瘀含药血清抗白血病细胞及白血病多药耐药细胞的体外实验.pdfVIP

• 828 • ( ) 中华中医药杂志 原中国医药学报 2011年4月第26卷第 4期 CJTCMP , April 2011, Vol . 26, No.4 ·研究报告· 解毒化瘀含药血清抗白血病细胞及白血病多药 耐药细胞的体外实验研究 姚宇红，严鲁萍，李秀军，高蕾 （贵阳中医学院第二附属医院血液科，贵阳 550003） 摘要： 目的：以解毒化瘀法立意，选取解毒化瘀中药复方，拟通过体外细胞培养证实其是否存在抗白血病 细胞及多药耐药白血病细胞的作用。方法：采用MTT比色法，选择白血病细胞K562、HL-60及阿霉素诱导的白血 病多药耐药细胞株K562/A、HL-60/A为靶细胞，观察不同浓度解毒化瘀含药兔血清对其细胞毒作用。结果：解毒 化瘀含药兔血清对K562、HL-60及K562/A、HL-60/A均有明显的抑制作用，且对其细胞毒作用呈剂量依赖性。结 论：解毒化瘀中药在抑制白血病细胞生长及白血病多药耐药细胞生长方面具有深入研究的价值。 关键词：解毒化瘀；白血病；多药耐药；体外细胞培 基金资助：贵州省中药管理局2008年资助项目 In-vitro study of resistant effectiveness of Jiedu Huayu medicated serums in leukemia cells and multidrug resistant leukemia cells YAO Yu-hong, YAN Lu-ping, LI Xiu-jun, GAO Lei ( Department of Hematology, the Second Affi liated Hospital of Guiyang College of TCM, Guiyang 550003, China ) Abstract: Objective: This study, through in-vitro culture of cells, testifies the effectiveness of the Chinese medicine Jiedu Huayu compound recipe in resisting leukemia cells and multidrug resistant leukemia cells. Methods: Utilizing the MTT colorimetry, this study chooses leukemia cell K562, HL-60, the doxorubicin-induced multidrug resistant leukemia cells line K562/A and HL-60/A as the cell targets in order to observe the effects, of Jiedu Huayu medicated rabbit serums at different density levels, on their cytokines. Results: Jiedu Huayu medicated rabbit serums apparently inhibits leukemia cell K562, HL-60, the doxorubicin-induced leukemia cell line K562/A and HL-60/A. In addition, it shows dose-rate dependence on the cytokines of K562/A. Conclusion: It is worthwhi