消痰散结方对人胃癌MNK-45荷瘤鼠机体HK-Ⅱ表达的影响.pdfVIP

1841 现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.10 MAY.2011 · · 消痰散结方对人胃癌MNK-45 荷瘤鼠机体HK- Ⅱ表达的影响 1 2△ 2 2 2 魏 振 魏品康 巨大维 张慈安 周 伟 (第二军医大学长征医院中医科 上海 200003) MNK-45 - ⅡHK- Ⅱ 摘要 目的：观察消痰散结方对人胃癌 荷瘤鼠糖酵解相关蛋白己糖激酶 （ ）表达的影响，探讨该方抗肿瘤机理。 30 10 MNK-45 方法： 只小鼠随机分为生理盐水组、消痰散结方组和希罗达组，每组 只。建立人胃癌 皮下移植瘤模型。灌胃治疗六 周后，称取各组瘤质量，计算抑瘤率和瘤体积。酶联免疫吸附测定法检测荷瘤鼠血清中HK- Ⅱ的水平；免疫组化方法检测瘤组织 中HK- Ⅱ蛋白的表达；结果：消痰散结方可明显抑制裸鼠人胃癌MNK-45 皮下移植瘤生长，下调荷瘤鼠血清及瘤组织中HK- Ⅱ表 达水平。结论：降低荷瘤鼠血清和组织中HK- Ⅱ的表达，抑制荷瘤鼠机体糖代谢过程可能是该方抗肿瘤作用的机制之一。 关键词：消痰散结方；肿瘤；糖酵解；小鼠 R735.2 R285.5 A 1673-6273 2011 10-1841-03 中图分类号： ， 文献标识码： 文章编号： （ ） Effects of Eliminating Phlegm Recipe on HK- ⅡExpression of MNK-45 Human Gastric Tumor-bearing Mice 1 2△ 2 2 2 WEI Zhen , WEI Pin -kang , JU Da-wei , ZHANG Ci-an , ZHOU Wei (Dep artment of Traditional Chinese Medicine,Changzheng hosp ital Second Military Midical University,Shanghai 200003,China) ABSTRACT Obj ective: To explore the antitumor effect of eliminating phlegm recipe on MNK-45 human gastric tumor-bearing mice. Methods: Thirty Kunming mice were randomly divided into normal saline (NS) group, Xiaotan Sanjie Recipe group and Xeloda group, ten for ench.Tumor-bearing models were established by injecting human gastric cancer MKN-45 injected into the subcutaneous site of BALB/c/-nu/nu mice. The mice were administered with medicine for six weeks. Weight of tumor xenografts were measured. tumor inhibiton rate