细胞培养课程.pptVIP

* * Cell Culture and Cell Isolation Basic Techniques for Mammalian Cell Tissue Culture Cell Isolation Ruijun Zhang Institute of Immunology in USTC Basic Techniques for Mammalian Cell Tissue Culture Cell subculturing Cell freezing Cell recovering Cell counting Cell subculturing Trypsinizing and Subculturing Cells From a Monolayer Passaging Cells in Suspension Cultures PROCESS Remove all medium Washing cells with Ca2+ Mg2+-free PBS Trypsin/EDTA solution to dislodge cells Wash cells and count the cells number Complete medium to inhibit the Trypsin activity Ad