PGBL论文：PGBL LPS RAW26 4.7细胞 TNF-α IL-6 NF-κB.docVIP

【关键词】PGBL LPS RAW264.7细胞 TNF-α IL-6 NF-κB 【英文关键词】PGBL Inflammation TNF-α IL-6 NF-κB PGBL论文：银杏叶多糖的抗炎活性及其抗炎信号转导机制 【中文摘要】本研究通过建立脂多糖（LPS）诱导的RAW264.7炎症细胞模型，检测了银杏叶多糖(PGBL)对炎症细胞的增殖活性、前炎症细胞因子及相关基因表达的影响；并通过检测PGBL对炎症细胞内核转录因子-κB的作用，探讨了PGBL在NF-κB炎性信号通路的作用，为PGBL的抗炎药物研发及临床应用提供理论与实验依据。方法：建立LPS诱导的RAW264.7炎症细胞模型，利用MTT法检测PGBL对炎症细胞RAW264.7增殖活性；双夹心ELISA法测定前炎症细胞因子TNF-α的表达；RT-PCR法测定前炎症细胞因子基因TNF-α、IL-6的mRNA表达；Westernblot法测定核NF-κB在细胞内的表达。结果：成功建立了RAW264.7炎症细胞模型；在特定浓度范围内，PGBL可显著抑制炎症细胞RAW264.7的增殖能力，且对RAW264.7细胞无毒性作用；PGBL可抑制炎症细胞RAW264.7的TNF-α的表达，且抑制率与PGBL浓度呈正相关；PGBL对炎症细胞RAW264.7的TNF-α和IL-6mRNA的表达有抑制作用，且抑制率与PGBL浓度呈正相关；PGBL对炎症细胞RAW264.7的NF-κB表达有抑制作用。结论：PGBL可以抑制炎症细胞RAW264.7的增殖，且对正常RAW264.7未表现出毒性作用。PGBL可通过调节炎性信号转导通路中NF-κB的表达，抑制TNF-α和IL-6基因的表达发挥抗炎活性。 【英文摘要】Ginkgo biloba extract is one of the most important part of active ingredient intraditional Chinese dedicine, in clinical, it showed anti-fatigue, anti-oxidation, protectliver, improving blood circulation and a variety of other pharmacological effects. LPSis the main component of the cell wall of Gram-negative bacteria, LPS-mediatedactivation of macrophages leads to the production of various cytokines such as tumournecrosis factor-α (TNF-α), interleukin-6(IL-6) and inflammation mediators such asnitric oxide (NO) and prostaglandin E2(PGE2). The production of these cytokinesmay result in the systemic inflammatory response syndrome (SIRS), severe tissuedamage, and septic shock. So lipopolyssacharide (LPS)-activated macrophages havetypically been used to evaluate the anti-inflammatory effects of various materials.In order to improve the medicinal value of Ginkgo biloba， and further expandthe scope of application of ginkgo biloba in clinical. In this paper, we study theanti-inflammatory activity of PGBL deeply. We built in vitro inflammatory model byLPS-stimulated murine RAW264.7macrophages, and further exploredanti-inflammatory molecular mechanism of PGBL by inflammatory signaltransduction pathway. The results showed that the r