冬凌草多糖论文：冬凌草多糖的提取、分离及初级结构研究.docVIP

冬凌草多糖论文：冬凌草多糖的提取、分离及初级结构研究 【中文摘要】本文以冬凌草(Rabdosia rubescens (Hemls.)Hara)为原料,研究了冬凌草多糖的提取、分离、纯化、理化性质、纯度鉴定、初级结构,并对4种冬凌草多糖组分的体外抗氧化活性进行了测定。主要研究内容与结论如下：1.对冬凌草多糖(RRPS)的提取工艺进行了较为系统的研究。通过单因素试验确定了冬凌草多糖的提取条件：料液比(1：10),热水(80℃)提取6h后,1%氢氧化钠提取6h；采用等电点法对RRPS进行脱蛋白处理；对RRPS的基本理化性质进行了测定。2.采用离子交换色谱和凝胶排阻色谱相结合对RRPS进行分离纯化,得到八个多糖组分。对其中的STPS-Ⅱ-a、STPS-Ⅱ-b、JTPS-Ⅱ-a和JTPS-Ⅱ-b四个组分进行纯度鉴定、分子量测定、理化性质及单糖组成测定。结果表明四个组分纯度均较高,分子量依次为39.1 kD、19.8 kD、26.2 kD和13.7 kD；糖含量分别为70.5%、70.3%、85.1%和82.3%；糖醛酸含量分别为10.5%、12.3%、9.8%和8.3%；蛋白质含量分别为5.5%、4.7%、4.3%、4.5%；采用PMP衍生后高效液相色谱法分析其单糖组成结果表明：组分STPS-Ⅱ-a和STPS-Ⅱ-b中的单糖组成相近,主要含有Gal、Man、GalUA和Ara四种单糖；组分JTPS-Ⅱ-a和JTPS-Ⅱ-b中的单糖组成相近,主要含有Xyl、Gal、Rha和GlcUA四种单糖。3.通过采用部分酸水解、全水解、甲基化反应、红外光谱、液相色谱、气相色谱-质谱、核磁共振波谱等方法对JTPS-Ⅱ-a的结构进行了较为系统的研究,初步确定：JTPS-Ⅱ-a的主链是由Ara、Xyl和Rha构成,GlcUA位于支链上,其分支位于Rha的碳二位上。4.通过清除DPPH自由基、羟基自由基、超氧阴离子自由基和抗脂质过氧化能力等一系列体外实验,考察了四种冬凌草多糖组分的体外抗氧化活性。结果表明：STPS-Ⅱ-a和STPS-Ⅱ-b对DPPH自由基和羟基自由基有较强的清除能力,JTPS-Ⅱ-a和JTPS-Ⅱ-b对这两种自由基的清除能力相对较弱。在所测定的浓度范围内,STPS-Ⅱ-a、STPS-Ⅱ-b、JTPS-Ⅱ-a和JTPS-Ⅱ-b均没有清除超氧阴离子自由基的能力,并且均没有抗脂质过氧化能力。 【英文摘要】In this dissertation, research of extraction, isolation, purification, physicochemical properties, homogeneity and structure of Rabdosia rubescens polysaccharid were made. A series of assay of antioxidant activity in vivo was also carried among four Rabdosia rubescens polysaccharid with different molecular weight. The main contents and conclusions are listed as follows:1. The extraction process of Rabdosia rubescens polysaccharides was more systematic researched, such as the extraction conditions was determined(solid:liquid=1:10, extracted 6 h with 1%NaOH after extracted 6 h with hot water) through single-factor test. Method of isoelectric point was applied to removal of protein by studying, and physicochemical characteristics of RRPS were investigated.2. Compoents of polysaccharide (8) were obtained by a combination of ion exchange chromatography and gel permeation. Four kinds of polysaccharides STPS-Ⅱ-a. STPS-Ⅱ-b、JTPS-Ⅱ-a and JTPS-Ⅱ-b were proved with higher purities by HP