桅子论文：中药材栀子的化学物质基础研究.docVIP

桅子论文：中药材栀子的化学物质基础研究 【中文摘要】本文对中药材栀子的化学物质基础进行研究,并建立了较为完善的质量标准,具体内容如下：建立了栀子药材的三波长高效液相指纹图谱；采用液质联用技术对指纹图谱主要色谱峰定性分析,推测了13个化合物结构及裂解方式；对栀子药材中京尼平龙胆二糖苷、栀子苷、绿原酸、西红花苷Ⅰ、Ⅱ进行含量测定；并将内参校正法应用于栀子药材多成分定量分析,考察不同类化合物相对定量时校正因子的系统适用性,结果显示,分别以栀子苷、西红花苷Ⅰ为参照评价相对定量京尼平龙胆二糖苷和绿原酸时校正因子适用性较差(RSD5%)；而相对定量栀子苷、西红花苷Ⅰ、Ⅱ时,校正因子适用性良好(RSD5%),相对定量的结果与实测值无显著差异(RSD5%),对比研究栀子药材不同炮制品以及不同果实部位的指纹图谱和主要成分的含量。与生栀子、炒栀子相比,焦栀子化学成分较少且主要成分含量偏低；栀子籽仁和果皮的化学成分有明显区别。建立了中药材栀子的质量标准,采用薄层色谱法同时鉴别栀子中京尼平龙胆二糖苷、栀子苷、西红花苷Ⅰ3个成分；考察了28批栀子药材的水分、灰分、酸不溶灰分、醇浸出物的含量,计算95%置信区间,按95%置信区间的80%和120%规定下限和上限,则水分、灰分、酸不溶灰分不得高于7.9%、7.6%、0.2%,醇浸出物不得低于9.1%；建立了整体表征栀子药材质量的3波长指纹图谱；多成分定量分析了栀子中京尼平龙胆二糖苷、栀子苷、绿原酸、西红花苷Ⅰ、Ⅱ的含量,按95%置信区间的80%为下限,分别不得低于6.0mg·g-1、30.1mg·g-1、0.8mg·g1-、4.4 mg·g-1、0.7 mg·g-1； 【英文摘要】In this paper, chemical substance of tranditional Chinese medicine of Gardeniae Fructus (GF) was studied, and a comprehensive quality standard was established. Details are as follows:Three-wavelength fingerprint of GF was established. LC-MS technique was applied for qualitative analysis, and thirteen compounds were identified by combing with related literatures; Five components of Genipin-1-gentiobioside, Geniposide, Chlorogenic acid, CrocinⅠand CrocinⅡin GF were determined; Quantitative analysis of multi-components by internal reference (QAMIR) was applied in FG quality evaluation, System suitability of a internal standard to determine different kind compounds was test. Geniposide, crocinⅠcould be the internal reference to determine Geniposide, crocinⅠ,Ⅱ, respectively. System suitability results of correction factor (f) were good (RSD5%) and results of QAMIR and external standard method were of no significant difference (RSD5%).Fingerprint and five main components contents of different processed drugs and different parts of GF were studied comparatively. Result showed that Chao Gardeniae Fructus (CGF) had little difference from GF while Jiao Gardeniae Fructus (JGF) distinguished much. Specifically, JGF had less chemical compositions and their main components contents