水分亏缺胁迫对烟草叶绿素荧光和光响应特性的影响-西南大学.DOC

烟草生长、光合特性及品质对水分胁迫的响应研究 龚 婷1,4，刘善丽1，尹国瞩1， 何 丹2， 张庆平3，宗学凤4* 1.重庆医药高等专科学校，重庆 401331 2.重庆人文科技学院，重庆 401573 3.重庆市永川区农委，重庆402160 4.西南大学农学与生物科技学院，重庆400716 摘 要：以云烟97为材料,采用人工控制土壤含水量的方法设置了轻度和重度水分胁迫，研究烟草生长、光响应特性、叶绿素荧光及品质对水分胁迫的响应。结果表明：（1）水分胁迫抑制烟草植株的生长。（2）胁迫处理下净光合速率、气孔导度和蒸腾速率下降，但差异不显著。重度水分胁迫下叶片胞间CO2浓度显著上升，这表明在干旱条件下，烟草叶片光合作用的抑制受到气孔与非气孔因素的双重影响。（3）随水分胁迫程度增加，叶片光补偿点和暗呼吸速率上逐渐上升而光饱和点和最大净光合速率则持续降低。（4）胁迫的强度会在水分逐渐降低的时候不断增强，同时伴随着的还有初始荧光的变化，呈现出先降后升的趋势，而最大荧光以及PSⅡ光化学效率(Fv/Fm)、光化学猝灭和实际光化学效率不断降低而非光化学猝灭不断增加。这表示光合器官在轻度水分胁迫时没有受到影响，而重度胁迫则造成PSⅡ反应中心破坏。（5）水分胁迫处理后烟草化学成分发生改变，轻度水分胁迫的烟草品质协调性分值最高，达95.85，表明适当地进行烟草中的水分胁迫处理能够提高烟草品质。 关键词：烟草；品质；光合特性；水分胁迫 Effects of Water Stress on the Growth, Photosynthetic Characteristics and Quality of Tobacco GongTing1,4,LuiShanli1 , YinGuozhu1,HeDan2, ZHANG Qing-Ping3 ,Zong Xuefeng4 1Chongqing Medical andPharmaceuticalCollege;2Chongqing College of? Humanities?Science??Technology; 3Yongchuan District Agricultural Commission ; 4 College of Agronomy and Biotechnology, Southwest University Chongqing 400716, China. Abstract: In order to study the change of growth, light response, chlorophyll fluorescence and quality of tobacco ‘Yunyan 97’ under different level water stress, our study had set up mild and severe level of water stress which were carried out by manual control of the water content in soil. The results showed that: (1) Water stress inhibited the growth of tobacco plant; (2) The net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) decreased under water stress but not significantly, and intercellular CO2 concentration (Ci) increased significantly under severe water stress, these results indicated that the inhibition of leaf photosynthesis were influenced by stoma and non-stoma factors under dry conditions; (3) With the increase of water stress, the light compensation point (LCP) and dark respiration rate (Rd) risen gradually, but the light saturation point (LSP) and net photosynthesis rate (Pn) decreased by degrees; (4) With the increase of