芒果苷对大鼠心肌缺血再灌注损伤的保护作用及其机制研究.pdfVIP

• 198 • Drug Evaluation Research 第 39 卷 第 2 期 2016 年 4 月 芒果苷对大鼠心肌缺血再灌注损伤的保护作用及其机制研究 1 1 2* 徐 丽 ，张元媛 ，曹军平 1. 武警总医院 药剂科，北京 100039 2. 武警总医院 消毒供应科，北京 100039 摘 要：目的 探讨芒果苷（MGF ）预处理对大鼠心肌缺血再灌注（MI/R ）损伤的保护作用及机制。方法 SD 大鼠随机分 为 5 组，即假手术组、模型组和 MGF 高、中、低剂量（40 、20 、10 mg/kg）组，采用结扎左冠脉前降支30 min 再灌注 2 h 的方法制备大鼠 MI/R 损伤模型，于术前 7 d 开始 ig 给药。采用比色法测定血清肌酸激酶（CK ）和乳酸脱氢酶（LDH ）活 性，ELISA 法测定血清肿瘤坏死因子-α （TNF-α）和白细胞介素-1β （IL-1β）水平；染色法测定心肌梗死面积（MIS ）；取心 肌匀浆，比色法测定髓过氧化物酶（MPO ）活性。结果 与模型组比较，MGF 高、中剂量组 MIS 、血清 LDH 活性显著降 低（P ＜0.05 ）；MGF 各剂量组血清 CK 活性、血清 TNF-α和 IL-1β水平、心肌 MPO 活性明显降低（P ＜0.05、0.01 ）。结论 MGF 预处理通过抑制中性粒细胞浸润，下调 TNF-α和 IL-1β表达，抑制炎症反应，发挥对 MI/R 所致心肌损伤的保护作用。 关键词：芒果苷；心肌缺血再灌注损伤；肿瘤坏死因子-α；白介素-1β；髓过氧化物酶 中图分类号：R965 文献标志码：A 文章编号：1674 - 6376 (2016) 02 - 0198 - 04 DOI: 10.7501/j.issn.1674-6376.2016.02.008 Cardioprotective effects of mangiferin against myocardial ischemia/reperfusion injury in rats 1 1 2 XU Li , ZHANG Yuan-yuan , CAO Jun-ping 1. Department of Pharmacy, General Hospital of Armed Police Forces, Beijing 100039, China 2. Department of Sterilization and Supply, General Hospital of Armed Police Forces, Beijing 100039, China Abstract: Objective To investigate the protective effects of mangiferin (MGF) against myocardial ischemia/reperfusion (MI/R) injury in rats. Methods Rat model of MI/R injury was established by coronary artery ligation for 30 min followed by 2 h reperfusion. Rats were divided randomly into five groups: Sham group, model group, MGF 10, 20, and 40 mg/kg group. MGF was ig administered once daily for 7 d pre-operation. The CK and LDH activities in sera were detected by chromatometry and the levels of TNF-α and IL-1β in sera were examined by ELISA after reperfusion. The myocardial infarction size (MIS) was measured by NBT staining. T