hmgb1与notch1在乳腺癌浸润和转移中的作用及其相关性.docVIP

精品论文 参考文献 HMGB1与Notch1在乳腺癌浸润和转移中的作用及其相关性 冷红刚1 王力2 孙伟(通讯作者)1 　　(1山东省潍坊医学院附属医院甲状腺乳腺外科 潍坊 山东 261052) 　　(2陕西省泾阳县医院普通外科 咸阳 陕西 713700) 　　【摘要】 目的：在乳腺癌组织中分别检测HMGB1和Notch1蛋白的表达，分析其与临床病理特征之间的相关性，着重探讨乳腺癌浸润和转移过程中两者的意义。方法：采用免疫组化SP法检测HMGB1和Notch1蛋白在60例乳腺癌组织及20例癌旁正常乳腺组织中的表达情况，并分析两者与乳腺癌患者临床病理学特征的关系。结果：HMGB1和Notch1蛋白在乳腺癌组织中表达都高于癌旁正常组织(P＜0.05)。HMGB1蛋白主要表达于细胞核中，少量可见细胞质；Notch1蛋白主要表达于细胞质中。在乳腺癌组织中，HMGB1阳性表达率为76.67%（46/60），Notch1阳性表达率为80.00%（48/60）；在癌旁正常组织中，HMGB1阳性表达率为15.00%（3/20），Notch1阳性表达率为10.00%（4/20）。HMGB1和Notch1的表达情况与乳腺癌的淋巴结转移、组织学分级、临床分期，均有关(P＜0.05)，两者在乳腺癌组织中的表达呈正相关关系(r=0.414，P=0.000)。结论：HMGB1和Notch1可能在乳腺癌的发生、发展中起重要作用，两者的高表达有可能作为预测乳腺癌浸润、转移的指标。 　　【关键词】 乳腺癌；HMGB1；Notch1 　　【中图分类号】R73 【文献标识码】A 【文章编号】2095-1752（2015）15-0038-04 　　【Abstract】Objective To investigate the significance in the process of invasion and metastasis of breast cancer, through detecting the expression of HMGB1 and Notch1 protein in breast cancer, and analyzing correlation of its clinicopathologic characteristics. Methods Using immunohistochemical SP method to detect HMGB1 and Notch1 protein in 60 cases of breast cancer tissue and the expression in 20 cases of normal breast tissue adjacent to carcinoma, and analyze the relationship with clinicopathologic characteristics of breast cancer patients. Results HMGB1 Notch1 protein expression in breast cancer tissue were higher than normal tissue adjacent to carcinoma (Plt;0.05). HMGB1 protein expression mainly in the nucleus, a small amount in cytoplasm，but Notch1 protein were mainly expressed in the cytoplasm. In breast cancer tissues, HMGB1 positive expression rate was 76.67% (46/60), Notch1 positive expression rate was 80.00% (48/60); in normal tissue adjacent to carcinoma, HMGB1 positive expression rate was 15.00% (3/20), Notch1 positive expression rate was 10.00% (4/20). The expressions of HMGB1 and Notch1 in breast cancer were re1ated with the 1ymph node metastasis，histo1ogica1 grade and c1inica1 stage(Plt;0.05), the e