hplc法测定复方桔梗远志麻黄碱片ⅰ中甘草苷及甘草酸的含量.docVIP

精品论文 参考文献 HPLC法测定复方桔梗远志麻黄碱片Ⅰ中甘草苷及甘草酸的含量 王莉萍1 胡莉1 高红1 韦祎2 　　（1重庆科瑞制药（集团）有限公司 重庆 400060） 　　（2重庆市中药研究院 重庆 400065） 　　【摘要】 目的：建立高效液相色谱法测定复方桔梗远志麻黄碱片Ⅰ中甘草苷及甘草酸的含量。方法：色谱柱为Agilent 5 HC-C18，流动相为乙腈-0.05%磷酸溶液（梯度洗脱），流速为1.0ml/min，甘草苷的检测波长为276nm、甘草酸的检测波长为250nm，柱温为30℃，进样量为20?l。结果：甘草苷和甘草酸的质量浓度分别在4.8274～43.4468、34.5511～310.9597?g/ml范围内与各自峰面积积分呈良好线性关系（r=0.999 9、0.999 9）；精密度、稳定性、重复性试验的RSDle;0.9%；甘草苷和甘草酸的平均加样回收率分别为99.6%、98.4%，RSD分别为0.9%、1.1%（n=6）。结论：本方法简便，准确，精密度好，适用于复方桔梗远志麻黄碱片Ⅰ中甘草苷及甘草酸的含量测定。 　　【关键词】 高效液相色谱法；复方桔梗远志麻黄碱片Ⅰ；甘草苷；甘草酸；含量测定 　　【中图分类号】R927.2 【文献标识码】B 【文章编号】2095-1752（2015）22-0351-03 　　Determination of Liquiritin and Glycyrrhizic Acid in Compound Radix Platycodi Polygala Ephedrine Tablets Ⅰ by HPLC 　　Wang Liping1，Hu Li1，Gao Hong1，WeiYi2 　　(1.Chongqing kerui Pharmaceutical Croup Co. Ltd.，Chongqing 400060) 　　（2. Chongqing Academy of Chinese Materia，Chongqing 400065) 　　【Abstract】 Objective To establish a method for the content determination of liquiritin and glycyrrhizic acid in Compound radix platycodi polygala ephedrine tablets Ⅰ by HPLC. Methods The determination was performed on Agilent 5 HC-C18 column with mobile phase consisted of acetonitrile-0.05% phosphoric acid (gradient elution) at the flow rate of 1.0ml/min. The detection wavelength was set at 262 nm for liquiritin and 250nm for glycyrrhizic acid. The column temperature was 30℃. The sample size was 20 ?l. Results The linear range were 4.8274～43.4468?g/ml of liquiritin (r=0.999 9), 34.5511～310.9597?g/ml for glycyrrhizic acid (r=0.999 9) . RSDs of precision, stabillity and repeatability tests were le; 0.9%. The average recoveries were 99.6% (RSD=0.9% , n=6) and 98.4% (RSD=1.1% , n=6). Conclusions This method is simple and accurate with good precision .It can be used for the contents determination of liquiritin and glycyrrhizic acid in Compound radix platycodi polygala ephedrine tablets Ⅰ. 　　【Keywords】 HPLC determination Compound radix platycodi polygala ephedrine tablets Ⅰ