基于DNA条形码技术对苍南海域仔稚鱼的物种鉴定.docVIP

基于DNA条形码技术对苍南海域仔稚鱼的物种鉴定 李渊 张丽艳 张然 宋普庆 王良明 张立 林龙山 国家海洋局第三海洋研究所 海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室 福建海洋研究所 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 采用DNA条形码技术对苍南海域冬季采集的仔稚鱼进行物种鉴定, 以提高物种鉴定的准确性。所有仔稚鱼通过形态特征鉴定出10个形态种, 但经条形码技术可鉴定出11个有效种。序列相似性结果显示11个形态种中10种可鉴定到种的水平, 1种无法匹配到相应的物种。条形码间隙分析结果显示各物种的最小种间遗传距离均高于该物种的最大种内遗传距离, 存在明显的条形码间隙。由系统发育树可以看出同一形态种的不同个体聚为一支系, 各物种间能明显区分开。同时结合本次渔业资源现场调查结果, 发现11种仔稚鱼中仅有5种的成鱼出现在本次调查中, 剩余6种为新出现种类。可见, DNA条形码技术除了能提高仔稚鱼物种鉴别的准确性外, 还能对物种多样性调查起到一定的补充作用。 关键词： DNA条形码; 生物多样性; 仔稚鱼; 序列相似性; 条形码间隙; 作者简介：李渊 (1985-) , 男, 博士生, 主要从事海洋生态学研究。E-mail:liyuan@ 作者简介：林龙山 E-mail:linlsh@ 收稿日期：2016-10-09 基金：海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室开放基金项目 (MATHAB201603) Identification of Several Fish Larvae Based on DNA Barcoding in the Investigated Waters of Cangnan LI Yuan ZHANG Li-Yan ZHANG Ran SONG Pu-Qing WANG Liang-Ming ZHANG Li LIN Long-Shan Third Institute of Oceanographgy, SOA; Fujian Institute of Oceanography; Abstract： The investigated waters of Cangnan located in the south of Zhejiang province, where is an important spawning ground for many marine species.Fishery resources survey in this water was conducted by bottom trawling in the winter of 2016 and a large number of fish larvae were collected.To improve the accuracy of species identification, DNA barcoding based on COI gene was employed to identify fish larvae collected from the investigated waters.Ten morphospecies were identified based on morphological characteristics, while 11 species were identified by DNA barcoding analyses.After running blast searches in NCBI, a match of 98%100% was found in 96.59% of all individuals, a match of 92%98% was 2.27%, and 85%92% was 1.14%.Results of sequence similarity showed that ten of 11 species were identified to the species level, while one species was unmatched.There would be a clear overlap (barcoding gap) between intraspecific and interspecific variabilities in each species, as the smallest interspecific divergences were well below 2%, but the la