不同生物I型磷脂酶A2N-末端衍生肽抗菌作用的研究.docVIP

精品论文 参考文献 不同生物I型磷脂酶A2N-末端衍生肽抗菌作用的研究 陶廷婷 史晓雄 梁宁生(通讯作者) 　　（广西医科大学附属肿瘤医院药学部 广西南宁 530021） 　　【摘要】目的：研究人、猩猩、猪、小鼠、大鼠五种生物I型磷脂酶A2（phospholipase A2, PLA2）N末端衍生肽在体外的杀菌效应，了解它们的结构与功能的关系。方法:根据人、猩猩、猪、小鼠、大鼠五种生物I型PLA2N-端第1-15个氨基酸残基为模板，分别合成衍生多肽AVW、ALW、AVN（因人与黑猩猩、大鼠与小鼠I型PLA2N-端第1-15个氨基酸残基序列相同，最终合成3条衍生多肽）。采用琼脂铺板计数法，将不同浓度的多肽分别与革兰阳性菌金黄色葡萄球菌和革兰阴性菌大肠杆菌在37℃孵育2.5h，然后铺板并置于37℃恒温箱培养18～24h，记录每一块琼脂板上的菌落形成数，并计算多肽杀菌率。结果：衍生多肽AVW、ALW、AVN对革兰阳性菌金黄色葡萄球菌和革兰阴性菌大肠杆菌均有杀菌活性，AVW和AVN对金黄色葡萄球菌和大肠杆菌杀菌作用较强，ALW对G+菌及G-菌杀菌活性较弱。结论:不同生物I型PLA2N-末端衍生肽对G+菌和G-菌均有杀菌活性，而具有较多碱性氨基酸衍生肽杀菌活性较强。 　　【关键词】 I型PLA2 N-端衍生肽 杀菌活性 构效关系 　　【中图分类号】R919 【文献标识码】A 【文章编号】2095-1752（2014）12-0051-02 　　A Study on Bactericidal Activity of Group I Phospholipase A2 N-terminal Derived Peptides from Different Animals 　　TAO Ting-ting,SHI Xiao-xiong,LIANG Ning-sheng（Department of Pharmacy, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning ,530021） 　　【Abstract】 Object:To analysis and compare the bactericidal activity of the polypeptides derives from the N-terminal sequence of group I phospholipase A2 in Homo sapiens、Pan troglodytes、Sus scrofa、Rattus norvegicus and Mus musculus in vitro, to understand the relationship between the bactericidal activity of the derived peptide and molecular structure. Methods:According to the N-terminal 15 amino acid sequence of group I PLA2 on the five mammal , The AVW, ALW and AVN were synthesized (because of the sequence of group I PLA2 from the Homo sapiens and the Pan troglodytes，the Rattus norvegicus and the Mus musculus were the same ,so only 3 polypeptides were synthesized). Different concentrations of peptide incubated with Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) in a water bath at 37℃ for 2.5 h. The reaction solution mixed with agar solution and poured plates. The plates were incubated at a temperature of 37℃ for 18-24 h, then were took out to count the colony-forming units (CFU) of