芽胞表面展示技术研究进展.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2010, October 25; 26(10): 1404−1409 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2010 CJB, All rights reserved. 细胞工厂的构建技术 芽胞表面展示技术研究进展 1 2 1 徐小曼 ，王啸辰 ，马翠卿 1 250100 2 610065 摘 要: 芽胞表面展示技术作为微生物表面展示技术的一种，因所表达的异源蛋白无需经过跨膜过程及芽胞的抗逆性 等独特优势而备受研究者的关注。以下介绍了芽胞的生理结构和形成过程、芽胞表面展示系统构建原则及目前所构建 的芽胞表面展示系统种类。芽胞表面展示技术不仅在疫苗生产中应用广泛，在生物催化和细胞工厂研究领域也具有广 阔的应用前景。 关键词: 芽胞，芽胞表面展示，生物催化 Recent progress of the research on spore surface display 1 2 1 Xiaoman Xu , Xiaochen Wang , and Cuiqing Ma 1 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China 2 School of Life Science, Sichuan University, Chengdu 610065, China Abstract: Spore surface display is one of attractive microorganism surface display systems. With the advantage of resistance attribute and specific assembly pattern, the technology of spore surface display now is attracting more and more attention. According to the current reports and main achievements of spore surface display, the structure and assembly of spores, the principle for construction and some existing spore surface display systems were elaborated in this paper. Now with the unique property of spores, the technique is not only widely used in production of vaccines but also has great applied potential in the field of biocatalysis and cell-factory. Keywords: spore, spore surface display, biocatalysis (Surface display) ( [1] )