日本五针松感染松材线虫病的检验检疫及扩散风险.PDFVIP

日本五针松感染松材线虫病的检验检疫及 扩散风险 来燕学 （浙江省宁波市森林病虫防治检疫站） Quarantine inspection and proliferation risks of Japanese five-needle pine (Pinus Parviflora Sieb.et Zucc.) infected by pine wood nematode (Bursaphelenchus xylophilus (Steiner and Buhrer) Nikle) associated with other parasites and microbes . Lai Yanxue (Forest Pest Management Station of Ningbo City, Ningbo 315012, China) Abstract: To reveal the characteristic and the spread risk of Japanese five-needle pine infected by pine wilt nematode, the xylem of dead Japanese five-needle pine were collected through the nursery sampling and the nematode associated with other parasites and microbes were isolated from chips of the xylem soaking in water; the isolates were inspected under microscope; and then the larvae (roundhead wood borer) in the xylem of dead Japanese five-needle Pine were further identified under dissected microscope. When the larvae metamorphose into the beetle of longhorn, the species of the longhorn beetle were further identified and then the beetle was cut into piece which was inspected under microscope to find whether or what nematode was carried by the beetle. The result showed that 33% of the samples were infected by Bursaphelenchus xylophilus; and 33% of the samples were infected by B. mucronatus ; 66.7%, 83.3% and 100% of the samples were infested by other invasive protozoon, fungus and bacterium respectively. The larvae (roundhead wood borer) are the larvae of Monochumus alternatus , which are able to metamorphose into the beetle of Monochumus alternatus which carry the nematode that is dauerlarvae LIV of Bursaphelenchus xylophilus. The conclusion is that the