内脂素在急性坏死性胰腺炎并发肾损伤中的作用.docVIP

精品论文 参考文献 内脂素在急性坏死性胰腺炎并发肾损伤中的作用 蔡莉1 唐国都2 许志毅1 （1广西医科大学 广西南宁 530021） （2广西医科大学第一附属医院消化内科 广西南宁 530021） 【摘要】目的 观察大鼠急性坏死性胰腺炎（ANP）发病过程中肾脏组内脂素mRNA表达变化，探讨内脂素在ANP并肾损伤中的作用及意义。方法 64只SD大鼠随机分为假手术对照组(C组)、急性坏死性胰腺炎组(A组)，各组32只。A组以1.5%脱氧胆酸钠溶液逆行注入胰胆管建立ANP模型，C组仅翻动胰腺后关腹。各组分别于造模后6 h、12 h、24 h、48h时间点分批处死大鼠；采用逆转录-聚合酶链反应(RT-PCR)法检测肾组织内脂素mRNA表达；胰、肾组织行病理切片观察，同时行血清淀粉酶、IL-6检测。结果 A组6、12、24、48时点胰腺和肾脏病理评分、血清淀粉酶、IL-6、内脂素 mRNA表达较同时点C组显著升高（均Plt;0.5）。A组大鼠随病程延长肾脏组织中内脂素 mRNA表达逐渐增加，自6时起明显高于C组（均Plt;0.5），48h最高。结论 在ANP发病过程中内脂素的表达在肾脏组织中逐渐增加，可能参与了ANP相关肾损伤过程。内脂素在并ANP并肾损伤时的表达升高可能与疾病的严重程度有关。 【关键词】急性胰腺炎 肾损伤 Visfatin IL-6 【中图分类号】R656.1 【文献标识码】A 【文章编号】2095-1752（2012）09-0389-02 ROLE OF VISFATIN IN THE PATHOGENESIS OF AUTE PANCREATITIS-ASSOCIATED KIDNEY INJURY 【Abstract】Objective To investigate the expression of visfatin in kidney tissure in rat with acute necrotizing pancreatitis (ANP) associated with liver injury，and to explore the role of visfatin in the pathogenesis of ANP associated with kidney injury. METHODS Sixty-four male Sprague-Dawley(SD) rats were randomly and equally divided into sham operation group(group C) and ANP group(group A)．Rat of group A was reduced by retrograde injection of 1.5% sodium deoxycholate (1ml/kg) into the biliary-pancreatic duct． All rats were acrificed at 6 hours，12 hours，24 hours and 48 hours after corresponding operation.The level of serum amylase and serum IL-6 was detected．Pancreas and kidney were evaluate by pathological score. The mRNA expression of visfatin in kidney were detected by RT-PCR．RESULTS Compare with group C，The pathologic scores of the pancreas and kidney，the level of serum-amylase and IL-6 in serum，the expression of visfatin In group A were significantly increase at 6 hours，12 hours，24 hours and 48 hours after corresponding operation(P＜0.05). CONCLUSION The increased expression of visfatin may closely related to the development of kidney injury in acute necrotizing pancreatitis． 【Key words】acute