培美曲塞对人肺腺癌细胞放射敏感性的体外实验研究.docVIP

精品论文 参考文献 培美曲塞对人肺腺癌细胞放射敏感性的体外实验研究 张婧 　　（兰州军区乌鲁木齐军区总医院肿瘤科 甘肃 兰州 830000） 　　【摘 要】目的：探讨电子线照射、培美曲塞、培美曲塞+电子线照射对人肺腺癌SPC-A4细胞凋亡产生的影响。方法：用细胞培养技术培养人肺腺癌SPC-A4细胞，通过MTT比色法分析电子线照射组、培美曲塞组、培美曲塞+电子线照射组对SPC-A4细胞的毒性作用。采用流式细胞术AnnexinV-FITC／PI双染法分析细胞凋亡率。结果：体外细胞毒性实验证明电子线照射组、培美曲塞组、培美曲塞+电子线照射组均对SPC-A4细胞有体外杀伤作用。电子线照射组、培美曲塞组、培美曲塞+电子线照射组均有凋亡发生。与空白对照组相比，电子线照射组、培美曲塞组、培美曲塞+电子线照射组的细胞凋亡率，差异有统计学意义(F=235．76，Plt;0．01)，其中培美曲塞+电子线照射组凋亡作用最明显，凋亡率达(48．7plusmn;0.76)％ ，显著高于电子线照射组、培美曲塞组(Plt;0．01)。结论：电子线照射、培美曲塞、培美曲塞+电子线照射三种处理方法均可诱导人肺腺癌SPC-A4细胞发生凋亡，培美曲塞+电子线照射诱导人肺腺癌SPC-A4细胞的凋亡率明显高于培美曲塞及电子线照射。 　　【关键词】培美曲塞；电子线；人肺腺癌SPC-A4细胞 凋亡 　　【中图分类号】R734.2 【文献标识码】B 【文章编号】1003-5028（2015）8-0521-02 　　The effect of pemetrexed on radiotherapeutic sensitivity of lung adenocarcinoma cell 　　Zhang Jing 　　【Abstract】Objective In order to find out how to enhance the apoptosis rate of SPC-A4 cell.The effect of SPC-A4 cell apoptosis induced by electron rays or pemetrexed disodium or combination of pemetrexed disodium with electron rays was studied. Methods Observe cell growth inhibition by MTT.Detect cell apoptosis by flow cytometry Result electron rays or pemetrexed disodium or combination of pemetrexed disodium with electron rays could significantly reduce the growth of SPC-A4 cell. Electronic line irradiation group,the pemetrexed group, pemetrexed and electronic line irradiation group, the apoptosis rate, the difference was statistical significance (F=235.76, P lt; 0.01). The apoptotic effect of the The pemetrexed and electron irradiationmost was obvious, and the rate of apoptosis (48.7 + 0.76%) was significantly higher than that of the other group (P lt; 0.01).Conclusion apoptosis of lung cancer cell could be induced by combination of pemetrexed disodium and electron rays or only pemetrexed disodium or electron rays.A combination of pemetrexed disodium and radiation achieved better effect of apoptosis than pemetrexed disodium or electron rays.Pemetrexed disodium enhanced sensibility of electron rays. 　　【Key Words】Pemetrexed