基于L-FABPGFP转基因斑马鱼构建放射性肝损伤模型的初步探讨.docVIP

精品论文 参考文献 基于L-FABPGFP转基因斑马鱼构建放射性肝损伤模型的初步探讨 胡威 　　（遵义医学院附属医院胸部肿瘤科 贵州 遵义 563003） 　　【摘要】 目的：初步探讨以L-FABP10:GFP肝组织特异性转基因斑马鱼建立放射性肝损伤模型的可能性。方法：出生后5天的斑马鱼，给予“剂量爬坡梯度”放疗，连续观察5天，了解幼鱼放疗安全性及安全剂量；结合“剂量爬坡”试验的结果及临床实践，选择合适的剂量进行放疗，连续观察5天，了解放疗后斑马鱼肝脏荧光强度、面积和形态的变化。结果：“剂量爬坡”试验显示：0、2、6、10、20、30、40Gy/次条件下的放射处理对斑马鱼是安全的；接近临床实践剂量40Gy/次的放疗处理未见斑马鱼肝脏荧光强度、范围及形态的变化。结论：L-FABP:GFP转基因斑马鱼放疗后54h内无法通过监测肝GFP荧光变化了解放射性肝损伤情况，该模型的建立仍需要进一步探讨。 　　【关键词】 放疗；斑马鱼；肝损伤；模型 　　【中图分类号】R735.7 【文献标识码】A 【文章编号】2095-1752（2015）24-0017-03 　　Establishment of Radiation-induced Liver Injury Model with LFABP:GFP Transgene Zebrafish 　　Hu Wei。Department of Thoracic Cancer, Affiliated Hospital of Zunyi Medical College, Guizhou Province, Zunyi 563003,China 　　【Abstract】Objective To investigate the establishment of radiation-induced liver injury model with L-FABP:GFP transgene zebrafish. Methods 5 days post-birth zebrafishes were given Dose climing test radiation and to assess the radiational security and choose the safe doses 5 days later. Combinded the above results with clinical practices to choose appropriated radiational doses, continuously observed the strength, area and shape changes of fluorescence for 54 h after radiation. Results Dose climing test show that it is safe to radiate under 0，2, 6, 10, 20, 30, 40 Gy/F for Zebrafish. There were no significant strength, area and shape changes of fluorescence under the doses near to clinical practice dose (40 Gy/F). Conclusion The radiation-induced liver injury of L-FABP:GFP transgene zebrafish canrsquo;t be observed after radiation in 54 h by evaluating the live fluorescence changes. So, the model need further researches. 　　【Key word】Radiotherapy; Zebrafish; Liver injury; Model 　　原发性肝癌（HCC, hepatocellular carcinoma）以手术及介入治疗为主，放疗因其剂量限制性毒性，作用有限，但手术及介入治疗已遇到技术上的“瓶颈”，因而提高放、化疗疗效，是当前提高肝癌治疗水平的关键[1,2]。正常肝细胞对放疗敏感，低剂量的放射处理即可造成严重的肝损害[2]，但其机理不清，建立有效的放射性肝损伤模型是阐明放射性肝损伤机制，减轻放射性肝损伤，以及提高放疗剂量和改善放疗疗效的前提。本文通过L-FABP10:GFP肝转基因斑马鱼，初步探讨以其建立放射性肝损伤模型的可能性。 　　1.实验材料及方法 　　1.