桂枝茯苓胶囊治疗大鼠子宫肌瘤及其机制研究.pdfVIP

·362 · 现代药物与临床 Drugs Clinic 第 30 卷 第 4 期 2015 年 4 月 桂枝茯苓胶囊治疗大鼠子宫肌瘤及其机制研究 孙 兰 1, 2，宗绍波 1, 2，吕耀中 1, 2，李卓琼 1, 2，周 军 1, 2，王振中 1, 2* ，萧 伟 1, 2* 1. 江苏康缘药业股份有限公司，江苏 连云港 222001 2. 中药制药过程新技术国家重点实验室，江苏 连云港 222001 摘 要：目的 探讨桂枝茯苓胶囊对子宫肌瘤大鼠的治疗作用及其机制。方法 采用注射苯甲酸雌二醇和黄体酮复制大鼠子 宫肌瘤模型，造模大鼠按体质量随机分为模型组、他莫昔芬（枸橼酸他莫昔芬片 3.6 mg/kg ）组以及桂枝茯苓胶囊0.14、0.27、 0.54 g/kg 组，另设对照组。连续给药 8 周后处死大鼠，取子宫、脾脏、胸腺并计算其脏器系数，比较子宫病理评分和组织形 态，放射免疫分析方法检测血清中雌二醇（E2 ）、孕酮（P ）及子宫组织中表皮生长因子（EGF ）水平。结果 桂枝茯苓胶 囊显著降低大鼠子宫系数，减轻子宫壁平滑肌增生，显著降低血清 E2 和子宫组织 EGF 水平。结论 桂枝茯苓胶囊对大鼠子 宫肌瘤具有治疗作用，其作用机制可能与降低血清 E2 、子宫组织EGF 水平有关。 关键词：桂枝茯苓胶囊；子宫肌瘤；大鼠；作用机制 中图分类号：R285.5 文献标志码：A 文章编号：1674 - 5515(2015)04 - 0362 - 04 DOI:10.7501/j.issn.1674-5515.2015.04.003 Therapeutic of Guizhi Fuling Capsule on uterine leiomyoma in rat and its mechanism SUN Lan1, 2, ZONG Shao-bo1, 2, Lü Yao-zhong1, 2, LI Zhuo-qiong1, 2, ZHOU Jun1, 2, WANG Zhen-zhong1, 2, XIAO Wei1, 2 1. Jiangsu Kanion Pharmaceutical Co., Ltd, Lianyungang 222001, China 2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China Abstract: Objective To discuss the therapeutical effect of Guizhi Fuling Capsule on uterine leiomyoma in rats and its mechanism. Methods Uterine leiomyoma model in rats was made by injection estradiol benzoate and progesterone. The rats with uterine leiomyoma were randomly divided into model group, tamoxifen (Tamoxifen Citrate Tablets 3.6 mg/kg) group, and Guizhi Fuling Capsule (0.14, 0.27, and 0.54 g/kg) groups. There was also a control group. After treatment for eight weeks, the rats were killed by cervical dislocation. The organ coefficients of the uterus, thymus, and spleen were calculated. Pathological score and pathological changes of uterus tissue were detected. The levels of estradiol (E2), progesterone (P) in serum and epidermal growth fa