双酚a对三角涡虫djsod,djcat,djgpx基因及相关酶活性的影响.pdfVIP

水生生物研究, 2015年, 第4卷, 第11期, 第1-5页 Shuisheng Shengwu Yanjiu, 2015, Vol.4, No.11, 1-5 研究报告 Research Report 双酚A 对三角涡虫 , , 基因及相关酶活性的影响 DjSOD DjCAT DjGPx 邹杨建 吴素格 李娟 类艳贝 张秀芳* 山东理工大学生命科学学院发育与进化生物学实验室, 淄博, 255049 * 通讯作者, zhxfshd@163.com 摘 要 本试验旨在探讨环境内分泌干扰物双酚A (BPA)对涡虫抗氧化酶(SOD, CAT, GPx) 的活性及相关 基因表达的影响。采用不同浓度的BPA 处理涡虫48 h ，然后测定抗氧化酶活性，并应用半定量RT-PCR 技 术探讨相关基因的变化。在BPA 胁迫下，SOD 活性呈现上升趋势、CAT 活性表现出了先上升后下降的趋 势，GPx 活性总体上变化不大。随BPA 浓度的增加， mRNA 表达量上升，并呈现剂量-反应关系， DjSOD DjCAT 及DjGPx mRNA 表达量也呈现出明显的上调现象，但变化趋势并不同于酶活性。BPA 诱导涡虫的 氧化应激反应，改变抗氧化酶SOD、CAT 基因的转录水平。 关键词 双酚A, 三角涡虫, 抗氧化酶, 基因表达 Effects of BPA on Activity and Gene Expression of Antioxidant Enzymes in Planarian Dugesia japonica Zou Yangjian Wu Suge Li Juan Lei Yanbei Zhang Xiufang * Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049 * Corresponding author, zhxfshd@163.com DOI: 10.13417/j.gab.034.001184 Abstract To understand the effect of Bisphenol A (BPA) on antioxidant enzymes (superoxide dismutase SOD, cat- alase CAT and glutathione peroxidase GSH-Px) activity and the mRNA levels for the genes encoding these antioxid- ant proteins, we used planarian as experimental material to study the antioxidant enzyme activities and the mRNA levels for the genes encoding these antioxidant proteins, under stress of different concentration of BPA for 48 h, the increase of SOD activities were most visible, meanwhile the CAT activities were found to be elevated first and then to be declined with the concentration of BPA exposure. No significant effects on glutathione peroxidase (GSH-Px) activities were observed excluding 0.564 mol/L BPA exposure. Real-time quantitative PCR showed that the transcripts of DjSOD were up-regulated and showed dose-response relationship with the conce