尘肺患者全肺灌洗液某些白细胞介素指标检测的意义.docVIP

尘肺患者全肺灌洗液某些白细胞介素指标检测的意义 ? 472?中国职业医学2007年12月第34卷第6期ChinOccupMed,December,2007,Vo1.34,No.6 文章编号:1000—6486(2007)06—0472—03 尘肺患者全肺灌洗液某些 白细胞介素指标检测的意义 袁宝军,王冬梅,邹吉敏,李超 【调查与研究】 摘要:目的探讨煤矿工人尘肺患者灌洗液中白细胞介素一12p70(IL-12p70),IL一12p40,IL.18和IL.10水平及意义. 方法用酶联免疫吸附双抗体夹心法检测59例尘肺患者(其中煤工尘肺4o例,矽肺19例)灌洗波中IL.12.p70,IL. 12p40,IL一18和IL一10水平.结果煤工尘肺患者灌洗液IL一18水平随期别增加而明显下降;矽肺患者灌洗液IL.18水平 随期别增加而上升,差异均具有显着性(Plt;0.01和Plt;0.05).与Ⅱ,Ⅲ期煤工尘肺相比,Ⅱ,Ⅲ期矽肺患者灌洗液IL. 18水平明显升高,差异有显着性(Plt;0.01),IL一12p70,IL一12p40和IL-10水平在各组中均无明显改变(Pgt;0.05).与单 纯煤工尘肺比较,煤工尘肺并发肺气肿患者灌洗液IL-10水平下降,差异有显着性(Plt;0.05);矽肺及其并发肺气肿患者 灌洗液各项检测指标无显着改变.IL一12p70,IL一12p40和IL一10三者间呈正相关,与IL-18不相关.结论尘肺患者存在 异常免疫应答.检测尘肺患者全肺灌洗液IL一18和IL-IO水平可能对鉴别尘肺期别及判断疾病严重程度有一定价值. 关键词:尘肺;灌洗液;白细胞介素 中图分类号:R135.2文献标识码:B SignificanceofdetectingwholelunglavagefluidIL一12p70,IL-12p40,IL?18andIL-10inpneumocouiosispatients YUANBao-jun,WANGDong—mei,ZOUJi—min,etal (DepartmentofClinicalLaboratory,AttachedKailuanHospitalof NorthChinaCoalMedicalCollege,Tangshan063000,China) Abstract:ObjectiveToexplorethesignificanceofdetectingwholelunglavagefluidIL-12p70,IL一12p40,IL一18andIL一 10inpneumoeoniosispatients.MethodsLunglavagefluidlevelsofIL一12pT0,IL一12p40,IL一18andIL一10weredeterminedin 59pneumoeoniosispatients[4ocoalworkerspneumoconiosis(CWP)and19silicosis]byasandwichELISA.ResultsLuIlg lavagefluidlevelsofIL一18inCWPweredecreasedwitllthestagegoingup,butinsilicosisIL一18levelswereincreased.and thereweresignificantlystatisticaldifferences(Plt;0.01,Plt;0.05,respectively).ComparedwitllstageIIandIIICWP,lung lavagefluidlevelsofIL-18instageIIandIIIsilicosisweresignificantlyhigher(Plt;0.01);howevernosignificantdifference wasobservedinlunglavagefluidIL-12pT0,IL-12p40andIL一10levelsamongthedifferentgroups(Pgt;0.05).Lunglavagefluid levelsofIL一10inCWPcombinedwithemphysemapatientsweresignificantlylowerthanthoseinsimpleCWPpatients(Plt; 0.O1).IL-12p70,IL一12p40,IL-18andIL一10levelshadnosignificantdifferencebetweensilicosisandsilicosiscombinedwith emphysemapatients.TherewerepositivecorrelationamongIL一12p70,IL一12raoand