一株丁草胺降解菌的分离鉴定及其降解特性的研究-微生物学报.pdf

微生物学通报 AUG 20, 2009, 36(8): 1178~1182 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 一株丁草胺降解菌的分离鉴定及其 降解特性的研究 1 1 1 1,2* 李艳春 熊明华 肖 晶 李春艳 (1. 150030) (2. 150001) 摘 要: 利用富集培养技术从长期施用丁草胺的稻田土壤中分离得到能够降解丁草胺的细菌1 株, 标记为 LYC-1 。经形态特征、生理生化特征和 16S rRNA 序列分析, 将该菌株鉴定为不动杆菌属 (Acinetobacter sp.), 菌株LYC-1 的最适生长温度为30°C, 最适pH 值为7.5 。当接种量为5%时, 该 菌株在含100 mg/L 的丁草胺无机盐基础培养液中培养7 d 后, 可使丁草胺降解达80%以上。 关键词: 丁草胺, 不动杆菌属, 生物降解, 分离鉴定, 降解特性 Isolation and Characteristics of a Butachlor Degradation Bacterium LI Yan-Chun1 XIONG Ming-Hua1 XIAO Jing1 LI Chun-Yan1,2* (1. College of Resource and Environment, Northeast Agricultural University, Harbin, Heilongjiang 150030, China) (2. Post-doctoral station of Northeast Forestry University, Post-doctoral workstation of Heilongjiang Academy of Agricultural Science , Harbin, Heilongjiang 150001, China) Abstract: One Butachlor-degrading bacterium named LYC-1 was isolated from a soil sample by enrichment culture. Combining morphologic characteristics and physiobiochemical characteristics with 16S rRNA se- quence analysis, strain LYC-1 was identified as Acinetobacter sp.. The optimal temperature and pH value for growth of strain LYC-1 was 30°C and pH 7.5 , respectively. The strain could degrade butachlor more than 80% when it was cultured in 100 mg/L butachlor medium for 7 days. Keywords: Butachlor, Acinetobacter sp., Biodegrading, Isolation and identification, Degrading characteristics (Butachlor) , ,