糖肾宁颗粒水提取部分工艺的研究-中药新药与临床药理.pdfVIP

·368 · 栽则葬凿蚤贼蚤燥灶葬造 悦澡蚤灶藻泽藻 阅则怎早 砸藻泽藻葬则糟澡 驭 悦造蚤灶蚤糟葬造 孕澡葬则皂葬糟燥造燥早赠，圆园14 May ，灾燥造援 圆5 晕燥援 3 糖肾宁颗粒水提取部分工艺的研究 尚雅文，李 晶，邹大威，龚慕辛，翟永松，高彦彬（首都医科大学中医药学院，北京 100069） 摘要：目的 筛选糖肾宁颗粒的提取工艺路线并优选水提部分工艺。方法 以KK-Ay 小鼠制备糖尿病肾病模 型，采用 联免疫法测定24 h 尿微量白蛋白含量，通过比较不同提取物给药前后微量白蛋白含量变化确定提 取工艺路线；水提部分以加水倍数、提取时间、提取次数为主要影响因素，大黄酸转移率为评价指标，采用正 交试验设计优化提取工艺条件。结果 路线2 降低尿微量白蛋白含量效果最好；优选的糖肾宁方水提部分最佳 提取工艺为加 10 倍水提取3 次，每次1.5 h 。结论 药效学试验确定了适宜的提取路线，优选的水提工艺条件 稳定可行。 关键词：糖肾宁颗粒；药效学实验；提取工艺路线；正交实验设计；水提 中图分类号：R284.1 文献标志码：A 文章编号：1003-9783（20 14）03-0368-05 doi 10.3969/j.issn.1003-9783.2014.03.033 Optimization of Water Extraction Process Technology for Tangshenning Granules SHANG Yawen ，LI Jing ，ZOU Dawei ，GONG Muxin ，ZHAI Yongsong ，GAO Yanbin （School of Traditional Chinese Medicine ，Capital Medical University ，Beijing 100069 ，China） Abstract Objective To screen the extraction routine and to optimize the water extraction process for Tangshenning granules. Methods The 24-hour microamount urinary albumin of KK-Ay mice diabetic nephropathy （DN ）model was detected by enzyme-linked immunosorbent assay （ELISA ）and was compared before and after administration of the extracts extracted by different technology routine. With the volume of water added ，extraction time and extraction frequency as the main influencing factor ，and with the rhein extraction rate as the evaluation index ，the water extraction process for Tangshenning granules was optimized. Results The No 2 extraction routine showed better effect on reducing microamount urinary albumin than the other two. The optimal water extraction process of No 2 extraction method was reflux with 10-fold water for 3 times ，extraction for 1.5 h per time. Conclusion Pharmaceutical screening can be applied to establish the appropriate extract process of Tangshenning granules ，and the optimized water extraction process is stable and feasible. Keywor