TLR7在结核分枝杆菌感染中的作用研究.pdfVIP

Chinese Journal of Zoono ses DOI 10 3969cjz issn 1002 2694 2014 12 011 j TL R7 T L R ssRNA T L R RAW T L R ssRNA RT PCR T ritonX ELISA ILT N F IL h RAW P h ssRNA h ssRNA ILP ILP ssRNA P h ILP T N F P T L R T L R ssRNA T L R ssRNA R378 91 A 1002 2694 2014 12 1223 04 Inhibitory effects of TLR7 on My cobacterium tuberculosis LI Ruif ang G U A N Zhi u F U Yurong YI Zhengun y j Teaching and Research Section We if ang M e dical Univ e rs ity We if ang China Dep artment of L aborato ry M e dic ine Shandong P rov inc ial K ey L abo ratory of Clinical L abo ratory D iag nos tics Clinical Faculty A f f iliate d H osp itaWe if ang M e dical Univ e rs ity We if ang China ABSTRACT T he aim of the present study w as to investigate the inhibitory eff ects of T L R on M y cobacte r ium tube rculos is T L R on infected RAW cells w as activated by chemical synthesis of T L R activation mo tif ssRNA Activated RAW cells w ere inoculated w ith M cobacte r ium tube rculos is uantitative PCR method w as applied to detect the phagocy tosis rate of y q cell to bacteria at diff erent time af ter infection Cy tokine production w as measured by ELISA f rom cell supernatant Cells w ere cultured on Roche medium and counted af ter sterile cracked w ith T ritonX and diluted w ith PBS Scanning electronic micro scope SEM w as applied to detect the morphological changes of cells treated w ith T L R