巯基苯类为标记分子免疫检测的FT2SERS光谱研究-上海有机化学.PDFVIP

2004 年第 62 卷 化 学 学 报 Vol. 62 , 2004 第 20 期 , 2118～2122 ACTA CHIMICA SINICA No. 20 , 2118 ～2122 ·研究简报· 巯基苯类为标记分子免疫检测的 FTSERS 光谱研究 李淑瑾　　顾仁敖 (苏州大学化学系 　苏州 215006) 摘要 　以对巯基苯甲酸、苯硫酚为标记分子,与金纳米粒子生成具有 SERS 信号的标记金溶胶. 在一定条件下 ,标记金溶胶 与抗体羊抗小鼠 IgG 形成 SERS 标记免疫金溶胶. 用组装金纳米粒子的玻璃表面吸附抗体 ,通过对相应抗原小鼠 IgG 的识 别 ,形成固相抗体/ 抗原对 ,再将 SERS 标记免疫金溶胶组装到固相抗体/ 抗原对上 ,形成“固相抗体 - 待测抗原 - 标记抗体 ( ) 即标记免疫金溶胶 ”夹心复合物 ,进而通过拉曼标记分子的 SERS 信号进行免疫检测. 关键词 　对巯基苯甲酸 ,苯硫酚 ,表面增强拉曼散射 ,金纳米粒子 ,免疫检测 Study on Fourier Transform SurfaceEnhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporterlabels LI , ShuJin 　　GU , RenAo ( ) Department of Chemistry , Suzhou University , Suzhou 215006 Abstract 　Gold colloids were labeled with Raman reporter mercapto molecules such as 4mercaptobenzoic acid and thiophenol to form Fourier transform (FT) surfaceenhanced Raman scattering ( SERS) reporterlabeled gold colloids. Firstly , antibody goat antirat was adsorbed onto the reporterlabeled gold colloids to form reporterlabeled immunogold colloids in appropriate condition. Secondly , the glass slide which was assembled gold nanoparticles was coated with antibody and selectively adsorbed antigen rat IgG to form antibodyantigen couple , and the SERS reporterlabeled immunogold colloids were