引物间同源性和裂口对PCR扩增的影响-生命科学研究.PDFVIP

4 1 生命科学研究 V ol . 4 N o . 1 2 0 0 0 3 L ife Science R esearch M ar . 2000 : 1007 -7847 ( 2000) 01-00 35 -0 6 PCR a 周　鹏 ( 57 1101) : 采用 PC/ G ene 软件对能扩增出特异带和不能扩增的 PCR 引物进行同源性 较 同 源间 “裂口( gap s) ”差数的统计 得出同源率小于或等于35% “裂口”差数大于或等于3 是获 得理想P CR 扩增效果的关键参数之一的结论 为引物设计提供了一条直观的依据. : P CR; 引物同源率; “裂口”差数; PC/ G ene 软件 : Q 7 81: A Effects of Homologization between Primers and Gaps on PCR Amplification ZHOU Peng ( N at ional K ey Biot echnol og y L aboratory f or T ropical Crops S CA T C Haikou 57 110 1 Hainan Ch ina) Abstract: Hom olog ous r at e w hich w as les s th an 35% ( inclu ding 35% ) and g ap s be- tw een prim ers w hich w as mo re t han 3 ( including 3) w ere the key facto rs of an ideal PCR amplification by u sing PC/ Gene sof tw are t o analyze t he ho molo gizat ion of am pli- fied or unamplified P CR prim ers . T his conclusion provided a st raight b asis f or primersp desig nat ion . : ; ; ; / Key words PCR prim ers ho molo giou s rate g aps P C Gene so ft w are PCR ( P olym er ase Ch ain React ion) PCR [ 13 8 9] . P CR . [ 2 3] . P CR [ 35 7] PCR PCR DESN PCR DESN A [ 2] 2 a : 1999 -09-17; : 1999-10 -08 : ( 39605) : ( 1963-) 15 T el : 0898-6988106 ( H) 6989226 ( O ) E -m ail : zhqt w @ public. hk . hi. cn . 2000 36 ( g ap s) PCR . 1 1. 1 ( Ly cop ersion esculentum ) T aq DN A dNT P s ( 100 mm ol/ L ) sigma .