芹菜素在静水压下对人体腰椎间盘髓核蛋白多糖核心蛋白基因表达的影响.PDFVIP

1 9 1 Vo.l19, No.1 2 0 1 1 1 OrthopedicJournalofChina Jan.2011 ExpermientalResearch · · ■ 柳根哲, 乔卫平, 徐　林, 李春根, 孙　旗, 曲　弋, 陈　江 (, 　 100700) [] 。 [] 32 32 , 1 3 1 ～ 2 mm , 1 ml DMEM 2.5 ml。 (Apigenin)、 G N -Monomethyl-L- arginine( L-NMMA) SodiumNitroprus- side( SNAP), 2 h, 、 , 。 [] 3 amt L-NMMA3 atm , (MOD)。 [ ] , mRNA。 :, 　, 　, 　, 　 : R6 87　　　: A　　　: 1005-8478 (2011)01-004 6-04 Effectsofapigeninonproteoglycancoreprotein sgeneexpression inthehumanlumbardiscnucleuspulposusin responsetohydrostaticpressure∥LIUGen- zh,e QIAOWei- ping, XULin, eta.lOrthopaedicCente,r DongzhmienHosp-i talAffliatedtoBeijingUniversityofChineseMedicin,e Beijing100700, China Abstrac:t[ Objective] Toinvestigatetheeffectsofapigeninonproteoglycancoreproteingeneexpressioninthehuman lumbarintervertebraldiscnucleuspulposusinresponsetohydrostaticpressure.[ M ethods] Thirty- twolumbarintervertebral discnucleuspulposusspecmienswereobtainedfrom patientswhohadundergoneposteriordiscectomy.Thesyringeswereplaced inawaterfilledpressurevesselkeptat37℃.Hydrostaticpressuresof1 (control), 3 and30 amtospheres(atm)wereap- plied.Afterincubationof2 hour,s eachnucleuspulposusspecmienwasembeddedinparaffin, slicedforsituhybridizationto studytheeffectoftheapigeninoftheintervertebraldiscnucleuspulposusproteoglycancoreproteingene stranscription.Asa G competitiveinhibitorofNO synt