中药蚓黄散冶对大鼠糖尿病溃疡创面形态及促愈因子的-环球中医药.PDFVIP

摇摇摇 10 环球中医药2016年1月第9卷第1期摇 Global Traditional Chinese Medicine,January2016,Vol郾9,No郾1 ·论著 · 中药“蚓黄散冶对大鼠糖尿病溃疡创面形态 及促愈因子的影响 李友山摇 杨博华摇 冀凌云 揖摘要铱摇 目的摇 应用中药“蚓黄散冶外治糖尿病足溃疡,观察其对创面组织愈合过程中的促进 作用,探讨大鼠糖尿病溃疡愈合的作用机制。 方法摇 建立大鼠糖尿病足溃疡模型后设模型组、西药 组、中药组各10 只,另设空白组10 只,空白组及模型组创面不予干预,西药组予甲硝唑注射液外敷, 中药组予“蚓黄散冶外敷,治疗 10 天后检测大鼠血清中晚期糖基化终末产物(advanced glycasylation end products,AGEs)、炎性因子及生长因子含量;增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)、CD34染色分别检测创面肉芽组织中成纤维细胞数、新生毛细血管数;HE染色观察组织形 态学变化。 结果摇 与模型组比较,药物干预后大鼠血清中AGEs和肿瘤坏死因子鄄琢、白细胞介素鄄1、 C-反应蛋白均可降低,碱性成纤维因子、表皮细胞生长因子、血管内皮生长因子、血小板衍生生长因 子水平均有所提高,创面肉芽组织中新生毛细血管数、成纤维细胞数均增多(P0.05),而中药组均 作用显著(P0.05)。 结论摇 “蚓黄散冶可明显促进大鼠糖尿病溃疡创面的愈合,其机制可能与改善 大鼠的炎性状态,提高大鼠血清中生长因子含量,降低AGEs水平,促进新生毛细血管、成纤维细胞 的增殖有关。 揖关键词铱摇 蚓黄散;摇 创面愈合;摇 炎性因子;摇 生长因子;摇 晚期糖基化终末产物 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2016郾01郾003 Effect of Yinhuangsan on morphology of ulcerwound andulcerhealingfactors on diabetic rats摇 LI You鄄shan,YANGBo鄄hua,JILing鄄yun.摇 Departmentof peripheralvascular,DongzhimenHospitalAffiliated to Beijing University of Chinese Medicine,Beijing 100700,China CorrespondingAuthor:LI You鄄shan,E鄄mail:lys4626@ 揖Abstract铱摇 Objective摇 To study the role of traditional Chinese medicines (TCM) “Yinhuangsan冶 in the external treatment of diabetic foot ulcers by observing the process of the wound tissue healing and exploring the mechanism of the rat diabetic ulcer healing. Methods摇 Diabetic foot ulcer model was established andtheratswererandomlydividedintomodelgroup,westernmedicinegroupandTCMgroup,10 rats in each group,ratsinall groupswererespectively by gavagegivenoral nodrug,metronidazoleand Yin鄄 huangsan. Another 10 normal rats were set as control group without drug treatment. After 10 days of treatment,thecontentsof AGEs,inflammatoryfactor andgrowthfactor in serumweredetected. Proliferating cell nuclear antigen (PCNA)