全基因组芯片筛查非小细胞肺癌组织多药耐药相关基因-Core.PDFVIP

322 · · 中国肺癌杂志2010 年 4 月第 13卷第 4 期 Chin J Lung Cancer, A pril 2010, Vol.13, No.4 ·临 床 研 究· 全基因组芯片筛查非小细胞肺癌组织 多药耐药相关基因 刘美燕 李春红 闫安 蔡莉 【摘要 】背景与目的 筛查与非小细胞肺癌多药耐药相关的基因，为非小细胞肺癌的个体化治疗提供理论依 据。方法 将手术切除的肺癌组织细胞进行原代培养。首先，采用MTT法检测诺维本、吉西他滨、多西他赛、紫杉 醇及顺铂对非小细胞肺癌组织细胞的抑制率和敏感性。再利用全基因组芯片筛选人高度敏感组和耐药组间的差异表 达基因。结果 共筛选出差异表达基因212个，与耐药组相比，高度敏感组中上调基因 168个，下调基因44个。结论 利用全基因组芯片筛查出212个可能与非小细胞肺癌多药耐药相关的基因，用于指导临床个体化治疗。 【关键词 】肺肿瘤；MTT法；全基因组芯片；多药耐药 R734.2 DOI: 10.3779/j.issn.1009-3419.2010.04.10 【中图分类号 】 cDNA Microarray Technique on Screening Multi-drug Resistance-related Genes of Human Non-small Cell Lung Cancer Meiyan LIU, Chunhong LI, An YAN, Li CAI Department of Medical Oncology, the Affiliated Tumor Hospital, Harbin Medical University, Harbin 150040, China Corresponding author: Li CAI, E-mail: caiwenxin76@ Abstract Background and objective The aim of this study is to screen for multi-drug resistance-related genes of 【 】 human non-small cell lung cancer (NSCLC), and provide the evidences for drug-sensitive predicting genes of different NSCLC patients treated with chemotherapeutic drugs. Methods Sensitivity and inhibition ratio of five antitumor drugs (NVB, GEM, TAL, DOC, CDDP) on 75 fresh NSCLC samples from different individuals were studied by means of culturing primary tumor cells and MTT assay. After the five chemotherapeutic drugs were used, multi-drug resistance-related genes of NSCLC with