星星草愈伤组织的诱导和继代培养.docVIP

星星草愈伤组织的诱导与继代培养 摘 要 本实验探索了不同培养基、外植体、激素浓度星星草诱导愈伤组织诱导的影响，以及pH与光照对星星草种子诱导愈伤组织的影响，激素浓度对星星草愈伤组织继代生长的影响。实验表明，星星草种子在MS培养基上愈伤组织诱导效果较好。星星草种子在含有2 mg/L 2,4-D，0.4 mg/L 6-BA的MS培养基上，诱导率可高达 87.5%； pH为5.8，6.0时，种子诱导愈伤组织效果比较好，平均诱导率分别为63.2%与61.48%。本实验还探索了光照对种子愈伤组织诱导的影响，完全避光培养时种子愈伤组织诱导率最高，为87%，随着透光孔数目的增多诱导率逐渐降低。星星草愈伤组织继代培养时2,4-D浓度为0.5 mg/L 1.0 mg/L时胚性愈伤组织率分别为92%，93%。 Induction of Puccinellia tenuiflora callus and subculture Abstract The experiment explored different media, explants, hormone concentration tenuiflora callus induction effects and pH and light on star grass seed callus induction effect, but also to explore the hormone concentration on callus tenuiflora subculture growth.The present study demonstrates Puccinellia tenuiflora seeds callus induction was better,on Murashige and Skoog (MS) basal media. The highest percentage of callus induction obtained from seed explants was 87.5% on Murashige and Skoog (MS) basal media supplemented with 2mg/L 2,4-D and 0.4 mg/L 6-BA; pH of 5.8,6.0, the seeds callus induction results were better, with an average induction rates were 63.2% and 61.48%. This experiment explored the effects of light on seed callus induction, completely avoid light culture seed highest callus induction rate was 87%, with a light hole, the increase in the number of induction rate decreased.The highest percentage of embryogenic calli obtained at 92% and 94% was observed on media containing 0.5 mg/L and 1.0 mg/L2,4-D,respectively. Keywords: Puccinellia tenuiflora; callus; medium; hormone; explant; Subculture 目录 摘要 3 第一章 综 述 5 1星星草概述 5 1.1星星草生物学特征及分布 5 1.2星星草的生态学特征 6 1.3星星草的栽培方法 6 1.4星星草应用 7 1.4.1星星草饲用价值 7 1.4.2星星草碱化草场 8 1.5星星草耐盐机制 9 1.5.1星星草种子的耐盐机制 9 1.5.2星星草根的耐盐机制 9 1.5.3星星草茎的耐盐机制 10 1.5.4星星草叶的耐盐机制 10 1.6星星草基因工程研究进展 11 1.6.1星星草耐盐基因研究进展 11 1.6.2星星草耐旱基因研究进展 11 1.7星星草应用、研究前景展望 12 1.8牧草生物技术育种研究 12 1.8.1我国牧草育种研究现状 12 1.8.2牧草生物技术育种研究应用与进展 13 2植物组培概述 13 2.1植物组培基本概念 13 2.2植物组培的常用培养基 14 2.3常用植物激素及配比 16 2.4植物组培应用研究 16 2.4.1快速繁殖 16 2.4.2无病毒苗培育 16 2.4.3培育新品种 17 2.4.4种质资源的保存 17