广藿香去油水提取物对雌雄小鼠肠推进功能的对比研究.docVIP

精品论文 参考文献 广藿香去油水提取物对雌雄小鼠肠推进功能的对比研究 梅清华1 陈燕1 兰树敏1 范翠琼1 钟淑秋2 （1广东省第二人民医院 510317；2广州中医药大学 510317） 【摘要】目的 探讨广藿香不同极性去油水提取物分别对雌雄小鼠肠推进功能的作用差别。方法　以水蒸气蒸馏法去除广藿香挥发油，水煎煮法得到水提取物，以极性4.3、5.4、6.6和10.2的不同溶剂提取，分别比较对雌雄小鼠肠推进功能的影响强度。结果　雌雄小鼠分别与阴性对照比较，雄性普遍表现出抑制趋势，且极性10.2差异有显著性（Plt;0.05）；雌性普遍表现出促进趋势，但差异无显著性（P＞0.05）；组内雌雄对比，极性10.2部位差异有显著性（Plt;0.05）。不分雌雄与阴性组比较，极性6.6部位抑制小鼠肠推进功能显著（Plt;0.05），极性10.2部位有抑制趋势（P＞0.05），而极性5.4和4.3部位有一定促进作用（P＞0.05）??结论　广藿香去油水提取物对小鼠肠推进功能有性别差异，雄性表现为抑制，雌性表现为促进。 【关键词】 广藿香 水提取物 肠推进 【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2013）26-0034-02 Effect of Water Extract from Herba pogostemonis on Intestinal Propulsion of Mice Mei Qing-Hua[1], Cheng Yan[1], Lan Shu-Min[1], Fan Cui-Qiong[1], Zhong Shu-Qiu[2] ([1]Guangdong №2 Provincial Peoplersquo;s Hospital;[2]Guangzhou University of Chinese Medicine, GuangZhou 510317) 【ABSTRACT】 OBJECTIVE To study the difference of water extract from Herba pogostemonis on intestinal propulsion of mice. METHODS Essential oil removed by steam distillation, water extract obtained by decocting, then extracted by solvents with different polarity of 4.3,5.4,6.6 and 10.2, compared the intestinal propulsion of mice. RESULTS Each compared with negative control, inhibition was observed on male mice, there was a significant difference in polarity 10.2 (P＜0.05);Promoting was observed on female mice, but there werenrsquo;t significant differences. Compared between male and female, there was a significant difference in polarity 10.2(P＜0.05).CONCLUSIONS There was gender difference of water extract from Herba pogostemonis on intestinal propulsion of mice, inhibition was observed on male mice and promoting was observed on female mice. 【KEY WORDS】 Pogostemonis Water Extract Intestinal Propulsion 广藿香为唇形科植物刺蕊草属植物广藿香Progostemon cablin（Blanco）Benth.的干燥地上部分，是常用的芳香化湿中药。近代药理研究表明，水提取物具有较显著的生理活性，比挥发油更能代表“和胃止呕”的功效，其不同极性再提取物对小鼠肠推进功能都有不同程度的抑制或促进[1,2，3]。我们在研究意外发现，去油水提取物对小鼠肠推进功能存在截然相反的性别差异，对雄性均表现为抑制，对雌性均表现为促进。