大肠杆菌还原制备金银纳米粒子的研究-中国科技论文在线.pdfVIP

中国科技论文在线 大肠杆菌还原制备金、银纳米粒子的研究# 陈国宝，杨洪英* （东北大学冶金学院，沈阳110819 ） 5 摘要：金、银纳米粒子因其独特的光线、电学、磁性和结构特性而被广泛应用于光电存储、 生物医疗、化工环境等领域。和传统的制备方法相比，生物制备方法具有良好的生物相容 性、反应条件温和、绿色且可持续发展等优点。利用大肠杆菌制备了金、银纳米粒子，采 用UV-Vis 研究了反应条件，TEM 与EDS 表征了材料的形貌和成分，并通过FT-IR 探究大 10 肠杆菌还原金银的机制。结果表明反应时间60 min 、贵金属前驱体浓度为1.0 g/L 时，大肠 杆菌还原所得的金、银纳米粒子平均粒径分别为10.82 nm 和 14.71nm 且大小比较均一。红 外分析结果表明大肠杆菌在还原贵金属前驱体时，多羟基化合物、蛋白质类物质等起还原 作用。 关键词：贵金属；纳米金；纳米银；大肠杆菌；生物合成 15 中图分类号：TF83 Biological Synthesis of Gold and Silver Nanoparticles by Escherichia Coli CHEN Guobao, YANG Hongying 20 (School of Metallurgy, Northeastern University, Shenyang 110819) Abstract: The gold and silver nanoparticles have been widely applied in many fields, such as optoelectronic data storage, biological treatments, chemical and environmental engineering, due to their unique optional, electronic, magnetic and structural properties. Using biological synthesis method displays more advantages, like biocompatibility of products, mild reaction conditions, 25 more green and sustainable. The gold and silver nanoparticles were prepared by Escherichia coli. The UV-Vis was used to study the reaction conditions, TEM and EDS have been employed to determine the shape and content of gold and silver nanoparticles. FT-IR was used to analysis the change of Escherichia coli solution before and after reduction. It shows that when the reaction time was 60 min, the concentration of precursor was 1.0 g/L, the obtained gold particles were 30