影响山羊体外受精的因素3.pdfVIP

动物学报 　50 (2) :216 - 221 , 2004 A cta Zoologica S inica 　　　　　 　 影响山羊体外受精的因素 周佳勃　吴延光　罗明久　韩　东　刘丽清　常仲乐　谭景和 山东农业大学动物科技学院, 泰安 271018 摘 　要 　以屠宰山羊卵母细胞为材料研究了公羊个体、附睾不同部位精子、成熟培养和受精时卵丘存在与否、 卵丘扩展程度及卵龄对山羊体外受精的影响。结果表明 : 1) 不同公羊精液在受精、卵裂和桑椹/ 囊胚率上都有 显著差异 ; 2) 附睾尾精子和鲜精的受精、卵裂和桑椹/ 囊胚率无显著差异 , 但显著高于附睾体和附睾头精子 ; 3) 成熟培养 24 和 27 h 卵母细胞的的桑椹胚/ 囊胚率显著高于培养 21 和 30 h 卵母细胞 ; 4) 卵丘扩展 3 和 4 级卵 母细胞受精和桑椹胚/ 囊胚率显著高于扩展 0 和 1 级卵母细胞 ; 5) 成熟培养前机械去卵丘严重影响卵母细胞体 外受精和桑椹胚/ 囊胚率 ; 6) 受精前完全去掉卵丘显著影响桑椹胚/ 囊胚率 [ 动物学报 50 ( 2) : 216 - 221 , 2004 ] 。 关键词 　卵母细胞 　精子 　体外受精 　卵丘 　山羊 Factors influencing in vitro fertilization in goats ZHOU JiaBo , WU YanGuang , L UO MingJiu , HAN Dong , L IU LiQing , CHAN G ZhongLe , TAN JingHe College of Animal Science and Technology , Shandong Agricultural University , Taian 　217018 , Shandong , China Abstract 　We studied the effect of buck individuality , epididymal origin of spermatozoa , presence and absence of cumu lus during in vit ro maturation and fertilization and length of maturation culture on in vit ro fertilization and embryo devel opment in goats. The results are summarized as follows : 1) semen from different bucks produced significantly different rates of fertilization , cleavage and morulae/ blastocysts ; 2) rates of fertilization , cleavage and morulae/ blastocysts by spermatozoa from cauda epididymidis were not different significantly from those of ejaculated spermatozoa but were markedly higher than those of spermatozoa from corpus epididymidis , and those of spermatozoa from co