植物生物技术导论目录.docVIP

植物生物技术导论目录 Preface to the Second Edition11081176268023537669 Preface to the First Edition AbbreviatIOns PART I：PLANT TISSUE CUL.TURE 1.INTRODUCTION New technologies Origin of biotechnology History 2 LABORATORY ORGANIZTION Washing facility General laboratory and media preparation area Transfer area Culturing facilities Light units Green houses Laboratory and pemonal safety 3.NUTRITlON MEDIUM Facilities and equipment Units for solution preparation Media composition Inorganic nutrients Carbon and energy source Vitamins Growth regulators Organic supplements Gelling agent pH Protocol：General methodology for medium preparation 4.STERILIZATION TECHNIQUES Preparation of sterile media，containers and small instruments Steam sterilization Units of energy and pressure Dry sterilization Filter sterilization Ultra violet sterilization Maintenance of aseptic conditions AIcohoI sterilization Flame sterilization Sterilization of explant ChemicaI sterilization Protocols Sterilization of seeds Sterilization Of buds，leaf，stem，roots，tubers，scales，etc. Sterilization of tissue for immature embryos，ovules and flower buds for anther culture 5.TYPES OF CULTURE CytodifferentIation Organogenic differentiation Types of culture Seed culture Embryo culture Mature embryo culture Immature embryo culture／embryo rescue Applications of embryo culture Callus culture Organ culture Nucellus culture Application Endosperm culture Application Cell culture Protoplast culture Protocols Protocol for seed germination（Nicotiana） Protocol for embryo culture（Cereals-wheat，maize，barley rice，etc.） Protocol for embryo culture（Legumes-green gram，black gram， french bean，soybean，etc.） Protocol for callus induction（Nicotiana tabacum） Protocol for callus induction（Cereals-wheat，rice，maize，barley,etc.） 6.MICROPROPAGATlON Axillary bud proliferation approach Meristem and shoot tip cultu re Bud culture Single node culture Axillary bud method OrganOgenesis Organogenesis via callus formation Direct adventitious organ formation