不同氮素水平对烟草碳氮代谢关键酶活性的影响.PDFVIP

18 中国烟草科学 2007 ，28 （1）：18-20，24 不同氮素水平对烟草碳氮代谢关键酶活性的影响 岳红宾 （河南省烟草公司许昌分公司，河南许昌 461000 ） 摘 要：用盆栽试验研究了不同氮素水平对烟草碳氮代谢关键酶活性的影响。结果表明，随着氮素水平的提高，淀粉酶活 性有升高趋势，烟叶硝酸还原酶活性呈上升趋势。低氮水平下由氮代谢转化为碳代谢的时间提前，高氮水平下由氮代谢转向 碳代谢的时间推后。综合认为中氮水平是生产优质烟叶的较好选择。 关键词：烤烟；碳氮代谢；淀粉酶；硝酸还原酶；转化酶；活性 中图分类号：S572.01 文献标识码：A 文章编号：1007-5119(2007)01-0018-03 Effects of Various Nitrogen Levels on Key Enzymes Activeness of Flue-cured Tobacco Leaves in Carbon and Nitrogen Metabolism YUE Hongbin (Xuchang Branch of Henan Tobacco Company, Henan Xuchang 461000) Abstract: The influence of various nitrogen levels on the activeness of key enzymes of flue-cured tobacco leaves in carbon and nitrogen metabolism were studied. The results showed that as nitrogen level increased the amylase activity enhanced, the nitratase activity enhanced. In low nitrogen level, the time from nitrogen metabolism to carbon metabolism was shorten; however, in high nitrogen level, the time was deferred. In summary, the mid nitrogen level should be the optimum level to attain the highest yield and quality of tobacco. Keywords: flue-cured tobacco; metabolism of carbon and nitrogen; amylase; nitratase; transferase; activity 碳氮代谢是烤烟植株最基本的代谢过程，与烟 1 材料和方法 叶品质形成密切相关。在烟叶生长成熟过程中，只 1.1 试验材料 有碳氮代谢平衡协调，才能生产出优质烟叶。而在 试验在华南农业大学农学院烟草基地大棚内 烟叶碳氮代谢过程中，各种酶的活性变化起着决定 3 性的调节作用，探讨碳氮代谢关键酶的活性变化规 进行。供试品种为 K326 ，土壤容重 1.27 g/cm ，pH 律对研究碳氮代谢对烟叶品质形成的作用机理有 7.25，有机质2.0%，全氮0.098%，速效氮 82.3 mg/kg， 重要意义。 速效磷 58.8 mg/kg，速效钾 85