家蚕内网虫属微孢虫核糖体小亚单位RNA(SSUrRNA)核心序列的克隆和序列分析.PDFVIP

动物学报 　49 (3) :414～420 , 2003 A cta Zoologica S inica 　　　　　 　 家蚕内网虫属微孢虫核糖体小亚单位 RNA ( SSUr RNA) 核心序列的克隆和序列分析 潘敏慧 　万永继　鲁　成　周泽扬　向仲怀 ( 西南农业大学农业部蚕学重点实验室 , 重庆 400716) Cloning and analysis of the core sequence of small subunit ribosomal RNA of Endoreticulatus bombycis PAN MinHui 　WAN YongJi 　L U Cheng 　ZHOU ZeYang 　XIAN G ZhongHuai ( ) Key Sericult ural L aboratory of A gricult ural Mi nist ry , Southwest A gricult ural U niversity , Chongqi ng 　400716 , Chi na Abstract 　Endoreticulatus bombycis is a new pathogenic microspordia isolated from the silkworm larvae. With polymerase chain reaction ( PCR) method , we amplified a fragment of the core sequence of the small subunit ribosomal RNA ( SSUr RNA) of Endoreticulatus bombycis . The SSUrRNA fragment was inserted into pMD18T Vector and then cloned. It had a length of 1 230 nucleotides and a percentage GC content of 513 %. The accession number in GenBank is g A Y009115. The secondary structure model of the SSUrRNA of Endoreticulatus bombycis was constructed both on the bases of the sequence alignment and RNAFOLD program of the PCGEN E package. The secondary structure model re vealed that Endoreticulatus bombycis lacked many eukaryotic helices , such as helix10 , helix 11 , helix 18 , helix 43 and helix 46 , but it has V4 region and has more of prokaryotic character. Analyzed by Blast , Endoreticulatus bombycis , En doreticulatus schubergi and Pleist