党参对鼠J 774巨噬细胞吞噬活性的增强效应.docVIP

党参对鼠J 774巨噬细胞吞噬活性的增强效应 党参对鼠J 774巨噬细胞吞噬活性的增强效应 更新日期：2011-01-24 点击： 贾泰元　Benjamin H.S. Lau 摘要：目的：验证党参对机体免疫功能的增强效应并探索其作用机理。方法：用党参水提液加入鼠J 774巨噬细胞培养液中作微量细胞培养，以自动微量荧光分析仪直接检测其对细胞吞噬活性的影响。结果：显示党参水提液对巨噬细胞的吞噬活性有明显的增强作用，在浓度500～3 000 μg/ml时，与无药对照组(O)比较都有显著性差异(P＜0.05)，分别增强1.33～1.70倍。结论：巨噬细胞是重要的免疫活性细胞之一，对机体免疫反应的各个环节都起关键性作用，党参能增强机体免疫的效应，可能是通过其增强巨噬细胞的免疫活性而实现的。 关键词：党参；　J 774巨噬细胞株；　氧爆炸 中图分类号：R285.5　　文献标识码：A　　文章编号：1008-0805(2000)09-0769-02 The Enhancing Effect of Chinese Medicine Radix pilosulae on J 774 Macrophage JIA Tai-yuan （Xian Central Hospital, Xian 710003, China） Benjamin H.S. Lau （Department of Microbiology, School of Medicine,Loma Linda University, Loma Linda, CA 92354 USA） Abstract：Objective: To probe into the mechanism of the enhancing effect of Chinese medicine Radix Codonopsis (CR) on organic immune activity. Method: The murine macrophage J 774 cell line was chosen to cultured with CR aqueous extract in different concentrations and then, the phagocytic activity was measured directly using a computerized microplate fluorometer. Result: The phagocytosis of macrophage J 774 was enhanced significantly by CR solution in concentrations 500 μg/ml to 3 000 μg/ml (P＜0.05). The peak value was at 2 000 μg/ml, which increased 170% compared with the control group. Conclusion: It is suggested that the mechanism of the enhancing effect of CR on organism is accomplished probably through the augment of macrophage function. Key words：Radix Codonopsis;　Macrophage J 774 cell line;　Oxidative burst 　　党参(Radix Codonopsis, CR)为桔梗科党参属植物，干燥根入药，可补气养血、增强机体免疫功能。中医用于“阴虚气弱、气虚”诸证，可以代人参，惟作用较弱［1］。为探究党参增强机体免疫功能的作用机理，我们用党参水抽提液加入鼠J 774巨噬细胞培养液中，观察其对该巨噬细胞吞噬活性的影响。现将结果报道于下。 1　材料与方法 1.1　药物来源及制备：实验用党参购自西安市药材公司，陕西产。原药烘干，磨细粉。称取干粉10 g，加蒸馏水100 ml，置56℃水浴箱温浴1 h，悬液经2 000 r/min离心15 min，上清液经0.45 μm醋酸纤维滤器(Corning Glass Works, Corning, NY, USA)过滤消毒。4℃冰箱保存备用。 1.2　巨噬细胞株：实验用J 774 A*1巨噬细胞株来源于BALA/C小白鼠网状细胞肉瘤［2］，由American Type Culture Collection CO. (Rockville, MD, USA)提供。 1.3　细胞培养及分析：J 774细胞在Dulbeccos改良Eagles培养液(Dul