乌贼墨糖胺聚糖的制备与理化性质研讨.pdfVIP

乌贼墨糖胺聚糖的制备与理化性质研究 1 1 2 1 1 李孝东 王凤山 宋允胜 曹吉超 徐斌 1.山东大学 药学院 生化与生物技术药物研究所 山东 济南 250012 2. 山东黄河医院 山东 济南 250032 摘要 本实验采用胰蛋白酶 链霉蛋白酶两次酶解 乙醇沉淀和 DEAE-纤维素离子交换柱分 离 从新鲜乌贼(Sepiella maindroni de Rochebrune)墨中提取得到乌贼墨糖胺聚糖 GAG 经 醋酸纤维素薄膜电泳和聚丙烯酰胺凝胶电泳证明乌贼墨GAG 为均一性多糖 紫外扫描无蛋白 质的特征吸收峰 红外光谱分析 样品具有典型的GAG 吸收峰 对该GAG 酸水解产物的薄 层色谱 纸色谱研究表明该GAG 主要由氨基半乳糖 葡萄糖醛酸 岩藻糖三种单糖组成 含 量分别为25.6% 29.2% 23.3% 硫酸根和蛋白质含量分别为8.2% 3.5% 关键词 乌贼墨 糖胺聚糖 制备 理化性质 Study on the preparation and properties of glycosaminoglycans from the ink of Sepiella maindroni de Rochebrune LI Xiao-dong, WANG Feng-shan, SONG Yun-sheng, et al. (Institute of Biochemical and Biotechnological Drug , School of Pharmacy, Shandong University, Jinan 250012, China) Abstract: One kind of glycosaminoglycans (GAG) was extracted and purified from the ink of Sepiella maindroni de Rochebrune by using the procedure of trypsin and pronase digestion, ethanol precipitation and DEAE-cellulose inon-exchange chromatography. It was proved to be homogeneous by polyacrylam ide gel and cellulose acetate membrane electrophoresis. No protein absorption was shown in its UV spectrum, but typical absorption of GAG was shown in the IR spectrum. It was identified by paper and thin layer chromatography of the acid hydrolyzate that the GAG was composed of galactosamine, glucuronic acid and fucose.The percentage content of galactosamine, glucuronic acid, fucose, sulfate and protein in the GAG was 25.6%, 29.2%, 23.3%, 8.2% and 3.5%, respectively. Key words : ink of Sepiella maindroni de Rochebrune; glycosaminoglycans; preparation; physical and chemical properties 通讯联系人 E-mail: fswang@sdu.edu.cn Tel: 0531-6652375