对荷瘤小鼠的抗肿瘤作用-中国科技论文在线.PDFVIP

《中国癌症杂志》2OO1年第ll卷第6期 CHINA ONCOLOGY 2001 Vol 11 No 6 GM—CSF基因修饰的肿瘤细胞疫苗 对荷瘤小鼠的抗肿瘤作用 [摘要] 目的：研究GM—CSF基因修饰的肿瘤细胞作为瘤苗的可行性。方法：通过逆转录病毒载体，将小鼠 GM—CSF基因导八EL-4淋巴瘤细胞中．研究ELd／GM—CSF在同系C57BL／6小鼠中的成瘤性及其诱导抗肿瘤免疫 的效果。结果：EL-4／GM—CSF细胞在小鼠中的成瘤性下降 50％小鼠无瘤生存 射线灭活的EL4／GM—CSF瘤苗能 有救地保护野生型肿瘤细胞的攻击，在肿瘤生长的早期对实验性荷瘤小鼠进行治疗，能延长荷瘤宿主的存活时间 (33．1±1．8)与对照组EL~／Wt(23．2±1．5天)比差异有显著性(P0 O1) 基因修饰的瘤苗作用明显增强：结 论：GM—CSF基因修饰的肿瘤疫苗可诱导较强的抗肿瘤免疫反应，导致肿瘤的部分根除，车实验为基因修饰的肿瘤 疫苗的临床应用提出r一定的实验依据： [关键词] GM—CSF：肿瘤疫苗； 基因治疗 中图分类号：R73—36 2 文献标识码：A 文章编号：1007—3639【20014 Anfitumor effect of GM·CSF gene modified murine EL_4 cell vaccine ZHANG Qing-yuan LI Dianqu~，州ⅣG z —hua，et at．r Department of Internal Medicine， ^ Theaching Hospital of HⅡ而in MedicaZ Uni~rsity,Hedon~ian Harbin 150040，China) [Abstract]Purpose：To study the vaccine potency of gene—modified tumor cells．Methods：The EL4 lympbema was Iransdueed with recombinant retlov Jrus containing the murlne GM—CSF gene．The effect of gene tr皿sduct】0n on ant Jtumor immunity was investigated Results：There aA,as no significant difference in the morpholo~．growth rate and expression of their surface marker between EL-4 cells and gene transduced EL4 ceils．GM-CSF gene transduced EL_4 cells resulted in remarkable loss of tumorigenicity in syngenetie mice EL~／GM—CSF cells could induce strong pmteefive immune reaction． Therapeutic vaccine of EL— GM—CSF cells could significantly retard the growth of established earl},一stage ELM／wt tumor． but did not retard the growth of late—stage EL-4／wt tumor Irradiated GM—CSF gene transduced EI _4 cells showed strmlg vaccine effect against challenged ELJ．cells Conclusions：GM-CSF gene transduced EL-4 ceUs