原子力显微镜技术在固相表面细菌吸附研究中的应用-应用与环境生物.pdfVIP

2010 16 4 ): 596~602 2010-08-25 应用与环境生物学报 ， ( Chin J Appl Environ Biol=ISSN 1006-687X DOI: 10.3724/SP.J.1145.2010.00596 原子力显微镜技术在固相表面细菌吸附研究中的应用* ** 吴华勇 黄巧云 蔡 鹏 梁 巍 荣兴民 (华中农业大学农业微生物学国家重点实验室，农业部亚热带农业资源与环境重点开放实验室 武汉 430070） 摘 要 原子力显微镜技术（Atomic force microscopy，AFM ）可用于定量研究微生物细胞的表面形貌及与固相表面间 的相互作用，并允许在近生理条件下直接测定细菌与固相表面间纳米水平的吸附力. 简述AFM基本操作原理，综述近 年来AFM在细菌吸附研究方面的细菌固定技术进展与挑战，非特异性相互作用如范德华力、静电力、疏水力、空间位 阻及表面粗糙度等及细胞表面聚合物如多糖、蛋白质、脂多糖等对细菌吸附的贡献，并提出该领域今后研究的特点 及应关注的问题 . 参 90 关键词 原子力显微镜技术；细菌吸附；吸附力；细胞探针；细菌表面聚合物 CLC Q93-31 Application of Atomic Force Microscopy (AFM) in Studies of Bacterial Adhesion * on Solid Surfaces WU Huayong, HUANG Qiaoyun**, CAI Peng, LIANG Wei Rong Xingmin (State Key Laboratory of Agricultural Microbiology, Key Laboratory of Subtropical Agricultural Resources and Environment, Ministry of Agriculture, Huazhong Agriculture University, Wuhan 430070, China) Abstract Atomic force microscopy (AFM) hasAtomic force microscopy (AFM) has ��ecome an important instrument for imaging of micromorphoecome an important instrument for imaging of micromorpho��ogy of microogy of micro��iaia�� ce�� surfaces with high reso�ution, and a technique for direct�y pro�ing nano-sca�e interfacia� forces of micro�ia� ce�� with so�id surfaces. This paper briefly introduced the basic operating principles of AFM. The advances and challenges on the attachment of bacterial cells to solid supports and AFM tips were reviewed. The contributions of nonspecific interactions such as van der Waa�s forces, e�ectrostatics, hydropho�icity, steric hindrance, surface ro