广玉兰提取物对铜绿微囊藻抑制作用机制研究.PDFVIP

33 5 2011 5 广玉兰提取物对铜绿微囊藻抑制作用的机制研究* 1, 2 1, 2 1 1, 2 1, 2 1, 2# 靳翠丽 周晓见 李 楠 缪 莉 董昆明 封 克 ( 1. , 225127; 2. , 225127) , ,, a ; ( SOD) ; , 7~ 8 , , : , , , a , SOD ; , 2 , , The inhibitory mechanism of Magnolia g randif lora linn extracts on Micr ocy stis aer ug inosa JI N Cuil i1, 2 , ZH OU X i 1, 2 1 1, 2 1, 2 1, 2 aoj ian , L I N an , M IA O Li , D ON G K unming , FEN G K e . ( 1. S hoo l of Environmental S ien e and Eng i neer ing , Yangz hou Univers ity , Yangz hou J iang su 225 127; 2. I nstitute of M ar ine S ien e and Te hnology , Yangz hou University , Yangz hou J iangsu 225127) Abstract: Tw o e tracts of M agnolia grandif lora linn, he ane e tract and butanol e tract, w ere found having st rong inhibitory effects on M i ro y stis aer ug inos a by our previous studies. In order to probe the anti algal mecha nism of t hese tw o e tracts, the content of chlorophylla, supero ide dismutase ( SOD) , membrane permeability and ultrastructure of M i ro y stis aerug inosa that treated by this tw o e tracts w ere investigated. The results showed t hat cont ent of chlorophylla w as declined rapidly , the SOD act ivity of algal cell w as slightly increased first and then pres ented a urgent decline, the membrane permeability was increased significantly , w ith t he highest permeability value was 78 time of that of control group. Accordingly , the ultrast ruct ure of alga cell w as also changed obviously , T EM phot ographs show ed that the cell was destroy ed severely w ith cell membrane w rinkled or even cracked, and pit h nu cleoid area was unobvious. The thylakoid lamella w ith linear structures was dispersed in cytoplasm messily . T he function of these tw o e tracts was different in alga inhibit ion,