聚氨酯泡沫固定化产碱杆菌细胞生物转化氰化物的研讨.pdf

2.5 环境化学工程/环境生物工程 聚氨酯泡沫固定化产碱杆菌细胞生物转化氰化物的研究 * 刘幽燕 ，李青云，陈敏，童张法 （广西大学化学化工学院环境工程系，南宁 530004） 摘要：利用1 株产碱杆菌DN25 作为降氰菌株，进行以聚氨酯泡沫为载体固定化及其转化特性的研究。结果表 明采用吸附生长法能有效实现菌株DN25 的固定，固定细胞量可达到每g 泡沫载体生物量干重0.35g。固定化 细胞的最适转化温度和pH 为35℃、8.0。对于低浓度氰化物，固定化细胞和游离细胞的转化速率相当；对于 高浓度氰化物，固定化细胞具有明显优势，不仅可耐受更高浓度的氰化物转化，其转化速率也高于游离细胞， 最大转化速率为507 mg /(L·h) ，是游离细胞的2.8 倍。通过初步的摇瓶模拟序列批式反应，固定化细胞活性 可保持20d. 关键词：聚氨酯泡沫；固定化细胞；氰化物；生物转化 中图分类号：X703.1 文献标示码：A 文章编号：0250-3301(2006) DEGRADATION OF CYANIDE BY AN ALCALIGENES SP. STRAIN IMMOBILIZED ON POLYURETHANE FOAM LIU You-yan, LI Qing-yun, Chen Min, TONG Zhang-fa (Department of Environmental Engineering, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China) Abstract: An Alcaligenes sp DN25 strain was immobilized on polyurethane foam and cyanide-biotransformation characteristics of immobilized cells were investigated in this study. It was found that the immobilization could be realized through the growth of the cell on the polyurethane foam and cell load of 0.35 gbiomass/gcarrier was obtained. The immobilized cell exhibited the best degradation behavior at pH 8.0 and 35℃. For the low-concentration cyanide, the degradation rate was the same no matter which the immobilized cell and the free cell were used, while the immobilized cell was proved to be of a more rapid degradation rate for the high-concentration cyanide. The highest cyanide-degradation rate for the immobilized cell could reach 507 mg CN-/L·h, which was 2.8 times as that of the free cell. Through the preliminary sequencing batch reactor, the immobilized cell could maintain active after 20 days. Key words ：polyurethane foam; immobilized cell; cyanide; biotransforma