气相色谱质谱和化学计量学组合方法分析半枝莲挥发性成分.docVIP

精品论文 参考文献 气相色谱质谱和化学计量学组合方法分析半枝莲挥发性成分 1常德市食品药品检验所 常德 415000；2衢州学院化学与材料工程学院 衢州 324000 【摘 要】目的：研究半枝莲挥发油成分。方法：利用水蒸气蒸馏法提取半枝莲挥发油，用气相色谱-质谱（GC-MS）检测，对重叠色谱峰采用直观推导式演进特征投影法进行分辨，从而获得每一组分的纯色谱和质谱，依靠每一组分纯质谱在NIST质谱库进行相似性检索而定性分析，用总体积积分法进行定量分析。结果：从半枝莲挥发油样品中共分离出71个色谱峰，鉴定出其中58个组分，占挥发油总含量的86.51%，主要组分为棕榈酸、甲氧基-4-乙烯基苯酚-十五烷酮、1-辛烯-3-醇、4- 甲基-1- 异丙基-3- 环己烯-1- 醇、丁香烯、氧化丁香烯、2-己烯醛和棕榈醛。结论：该方法不仅可鉴定的化合物数目增加，而且提高了定性准确度，可用于中药半枝莲质量控制，能为进一步研究和开发半枝莲提供有利的科学依据。 【关键词】气相色谱/质谱；直观推导式演进特征投影法；挥发油；半枝莲 [Abstract] Objective：To study the volatile components of Scutellaria barbata D.Don.Methods：The volatile chemical constituents were extracted by water distillation method and detected by GC-MS.After the overlapping chromatographic peaks were resolved into pure chromatograms and spectra with heuristic evolving latent projections（HELP），qualitative analysis was performed by similarity search in NIST library with the obtained pure mass spectrum of each component and the quantitative results were obtained by calculating the volume of total two-way response.Results：A total of 71 components were separated and 58 components were identified，accounting for 86.51% of the total content.The main components were n-hexadecanoic acid，6，10，14-trimethyl-2-pentadecanone，1-octen-3-ol，4-methyl-1-（1-methylethyl）- 3-cyclohexen-1-ol，caryophyllene，caryophyllene oxide，2-hexenal，hexadecanal and so on.Conclusions：Not only more components were identified by the established method，but also the qualitative analysis of components was improved to a reliable degree.The proposed approach can be used to quality control of Scutellaria barbata D.Don.The obtained results can also provide useful information for further study and development of Scutellaria barbata D.Don. [Keywords] GC-MS；Heuristic evolving latent projections；The volatile constituents；Scutellaria barbata D.Don 半枝莲（Scutellaria barbata D.Don），又名并头草、狭叶韩信草，是马唇形科黄芩属多年生草本植物，主产于浙江、江苏、安徽、河南等地 [1，2]。半枝莲性寒、味辛、苦，具有清热解毒、散瘀止血、利尿消肿等功效，用于治疗疔疮肿毒、咽喉肿痛、跌打伤痛、水肿、黄疸、蛇虫咬伤等，是民间常用中草药[3]，现代药理活性研究表明，半