nHAP类人胶原蛋白壳聚糖复合材料制备方法比较研究.pdfVIP

nHAP/类人胶原蛋白壳聚糖复合材料制备方法比较* 2 1 2 1 2 1 2 1 2 1 2 1 2 高琛 ，米钰 ，范代娣 ，朱晨辉 ，骆艳娥 ，马晓轩 ，惠俊峰 （1 陕西省可降解生物医用材料重点实验室，2 陕西省生物材料与发酵工程技术研究中心，陕西 西安） 摘要：中温下，采用两种不同的方法制备nHAP/重组类人胶原蛋白壳聚糖复合材料，第一种方法是通过将水热合成、重结 晶相结合制备的纳米羟基磷灰石浆料与类人胶原蛋白壳聚糖共混，制备复合材料；第二种方法是在重组类人胶原蛋白中原位 形成羟基磷灰石后加入壳聚糖溶胶制备复合材料。采用XRD、FTR、扫描电镜、ZETASIZER 粒径测试和力学性能测定等方法 对两种复合材料的特性进行表征，经比较分析发现：两种方法制备的复合材料在晶相组成、化学组成、纳米羟基磷灰石晶体 尺寸、胶原纤维的结构等方面均与天然骨相似。但与水热重结晶法相比，原位合成制备的纳米羟基磷灰石结晶度更接近自然 骨，由其制备的复合材料在均匀性和机械强度方面也优于共混法。因此，原位合成法是改善纳米羟基磷灰石/类人胶原蛋白 壳聚糖复合材料均匀性和力学性能的有效方法。 关键词：纳米羟基磷灰石（HAP ）；重组类人胶原蛋白(RHLC)；壳聚糖(CS)；可降解复合材料；研究方法 中国分类号：R318.08 文献标识码： 文章编号： A comparative study on preparation methods of nano-hydroxyapatite /Recombinant Human-like Collagen /chitosan composites 2 1 2 1 2 1 2 1 2 1 2 1 2 GAO Chen , MI Yu , FAN Dai-di , ZHU Chen-hui , LUO Yan-e , MA Xiao-xuan ,XI Jun-feng (1.The Key Lab in Biomedical Degradation materials,Shanxi,Xi’an 710069;2. Biomaterials and fermentation engineering center of shanxi, Xi’an, Shanxi 710069) Abstract: Composites were prepared by two methods of mixing hydrothemally recrystallisation treated nano-hydroxyapatite with Recombinant Human-like Collagen （RHLC ） /Chitosan( CS ) solution and in situ synthesis of hydroxyapatite in RHLCC solution at moderate temperature. The characteristics of the composites were analyzed by XRD, FIR and ZETASIZER. Mechanical properties of the composites were also tested A comparative study on the characteristics of two kinds of composites was carried out. The results showed that both the composite prepared by mixing method and the composite formed by in situ synthesis were nano-hydroxyapatite.However, the degree of crystallinity of hydr