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三孢酸及类胡萝卜素代谢通量的相互关系研讨.pdf

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J041 STUDIES ON THE RELATIONSHIP OF METABOLISM FLUX BETWEEN TRISPORIC * ACIDE AND CAROTENE ** *** LUO Mingxiang YUAN Qipeng ZHU Yan and Liu Zhen (Pharmaceutical Engineering Department , Beijing University of Chemical Technology, Beijing 100029, China) Abstract Trisporic acid, isolated and purified from mated Blakeslea trispora culture, its stability to heat, light, oxide-reducing agents was investigated. TA promoted the synthesis of lycopene when added back in the optimum condition. The effect of TA on lycopene metabolism flux was also approached in this paper. Evidence is presented that TA is sensitive to light, heat and reducer, but not to oxide agent. The extra addition of TA stimulated MVA and depressed IPP isomerase, enhanced metabolism flux, which then promoted the precursor of phytoene, made lycopene accumulate greatly. The synthesis of lycopene was significantly promoted by TA when added back at 11.1mg/L in the fourth day, and the amount of lycopene was promoted up by 44%. Keywords Trisporic acid, lycopene, metabolism flux 1 Blakeslea trisporaB.trispor a (+)(-) _______________________________________ * No ** :22 ***:75 100029Email yuanqp@mail.buct.edu.cn 1 1.1 B.trisporaATCCATCC 14271(+)ATCC 14272( ) 203 204 1.2